在两个多抗药性大肠杆菌 (Escherichia coli) 血统中,并行丧失了VI型分泌系统
Elizabeth A Cummins1, Robert A Moran1, Ann E Snaith1
1Institute of Microbiology and Infection, College of Medical and Dental Sciences, University of Birmingham, Birmingham, B15 2TT, UK.
Microbial genomics
|November 16, 2023
概括
多抗药性大肠杆菌克隆构成了全球健康威胁. 这项研究调查了VI型分泌系统 (T6SS) 是否有助于它们的肠道殖民,发现MDR克隆通常缺乏必要的T6SS基因.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 公共卫生 公共卫生
背景情况:
- 多药耐药 (MDR) *大肠杆菌*克隆是全球日益严重的公共卫生问题.
- 耐药的大肠杆菌可以取代人类肠道中的共生细菌,这表明了竞争优势.
- 第六类分泌系统 (T6SS) 是这种竞争优势的潜在机制,特别是在快速殖民的场景中.
研究的目的:
- 调查VI型分泌系统 (T6SS) 在多抗药性大肠杆菌 (MDR *E. coli*) 的竞争优势中的作用.
- 为了确定T6SS基因携带是否与E. coli*系遗传多样性中的MDR血统有关.
主要方法:
- 对超过20,000个大肠杆菌基因组进行了大规模的基因组分析.
- 遗传学分析以评估T6SS基因的分布.
- 在特定的MDR克隆 (ST410和ST131) 中T6SS位点的表征.
主要成果:
- 大肠杆菌中T6SS基因的存在显示出显著的非遗传学变异.
- 在T6SS基因携带和MDR谱系之间没有发现直接关联.
- 含有MDR克隆的多个细胞群表现出重要的结构T6SS基因的损失.
- 特定的遗传事件,包括重组和插入,被确定为导致MDR克隆ST410和ST131.1.中T6SS基因并行丧失的原因.
结论:
- 类型VI分泌系统 (T6SS) 并非普遍存在于MDR *E. coli*中,并且似乎不会通过其存在来赋予一般的竞争优势.
- 在某些MDR *E. coli*类中失去重要的T6SS基因表明了它们成功或适应的替代机制.
- 了解MDR *E. coli*中T6SS基因丢失的遗传基础对于理解它们的流行病学和公共卫生影响至关重要.
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