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抗微生物耐药性实验室网络对ThermoFisher Sensititre GN7F菌微稀板进行多站点评估,用于抗微生物敏感性测试

Amelia S Bhatnagar¹, María-José Machado¹, Logan Patterson²

¹Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention , Atlanta, Georgia, USA.

Journal of clinical microbiology

|November 16, 2023

概括

在抗微生物敏感性测试中增加转移量 (TV) 提高了对诸如Enterobacterales等多药耐药生物体的检测. 对于Sensititre GN7F面板建议使用30μL电视,以提高准确性和公共卫生监测.

科学领域:

临床微生物学临床微生物学
传染性疾病传染性疾病
抗微生物药物耐药性的监测和监测

背景情况:

疾病控制和预防中心 (CDC) 在2017年建立了抗菌素耐药性实验室网络,以加强国内检测多药耐药生物的能力.
准确的抗微生物敏感性测试 (AST) 对于指导临床治疗和针对耐药细菌的公共卫生策略至关重要.

研究的目的:

评估不同传输量 (TV) 对AST商用微稀释面板性能的影响.
为了确定Sensititre GN7F面板的最佳TV,以改善检测多药耐药生物体,特别是肠杆菌 (ENT),Pseudomonas aeruginosa (PSA) 和Acinetobacter baumannii (ACB).

主要方法:

评估了Sensititre GN7F面板,使用来自CDC和FDAAR隔离库的100个隔离物.
测试了四种传输体积 (1,10,30和50μL) 和四种电视组合 (STD,E-STD,30μL,50μL).
根据生物体和TV的评估基本协议 (EA),分类协议,重大错误 (ME) 和非常重大错误 (VME).

主要成果:

对于Enterobacterales,基本一致性为标准TV的15种β-乳酸盐中的7种<90%;增加的TV改善了EA (>90%) 并减少了非常严重的错误.
Pseudomonas aeruginosa 显示了随着电视的增加而改善的 EA,但也增加了重大错误.
Acinetobacter baumannii在增加电视时显示出轻微的EA改进,但所有测试卷都导致了多个主要和非常主要的错误.

结论:

建议将传输体积增加到30μL的Sensititre GN7F面板,特别是Proteeae部落 (P部落) 肠杆菌,以提高准确性.
这种调整,尽管是"非标签"的,但由于错过的抗菌素耐药性的重大公共卫生和临床后果,这种调整是合理的.
需要进一步优化AST方法,以提高对具有挑战性的生物体 (如Acinetobacter baumannii) 耐药性的准确检测.

关键词:

抗微生物耐药性抗微生物耐药性对抗微生物的敏感性测试.牛微稀释的方法商业的 AST 商业的 AST 评价评价评价评价

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