细胞RNA引导的内核酶是从一个独特的细菌酶类进化出来的
Peter H Yoon1,2, Petr Skopintsev2,3, Honglue Shi2,4
1Department of Molecular and Cell Biology, University of California, Berkeley; Berkeley, CA, USA.
Nucleic acids research
|November 16, 2023
概括
由RNA引导的内核酶,包括TnpB核酶,是从 prokaryotic 插入序列进化为真核 Fanzor 蛋白质的. 这项研究追踪了它们的进化路径,揭示了适应策略和基因家族进化的新模型.
科学领域:
- 分子生物学分子生物学
- 进化生物学 进化生物学
- 遗传学 遗传学 是一个
背景情况:
- RNA引导的内核酶对于生物过程至关重要,如适应性免疫和基因组编辑.
- 原核生物中的插入序列 (IS),例如IS200/IS605和IS607家族,编码TnpA转换酶和TnpB核酶.
- TnpB核酶是RNA引导的酶,也是CRISPR-Cas12酶的祖先.
研究的目的:
- 分析 prokaryotic TnpB 核酶与它们的真核同类之间的进化关系,Fanzors.
- 了解真核生物中IS607 TnpBs的进化轨迹和适应.
- 阐明TnpBs/Fanzors及其同类转换酶之间的共同进化策略.
主要方法:
- 对 prokaryotic TnpBs 和eukaryotic Fanzors 的遗传学分析.
- 一个IS607TnpB和Fanzor1蛋白质的生物化学表征.
- 酶结构和功能的比较分析.
主要成果:
- 两种Fanzor1和Fanzor2蛋白都来自IS607 TnpBs的单一血统,具有独特的活性位点.
- 范索斯的流行表明,这种IS607 TnpB血统在真核生物环境中的适应是有利的.
- 在TnpBs和Fanzors与它们各自的转换酶的共同演变中确定了共同的战略.
结论:
- 提出了一个从IS607 TnpBs到Fanzor2s,然后到Fanzor1s的顺序进化的新型模型.
- 这项研究详细介绍了 prokaryotic 基因进化为新的真核蛋白家族的过程.
- 突出了特定的 prokaryotic 基因系对真核系统的适应性.
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