对Mycobacterium tuberculosis多重耐药 (MDR) 菌株的分子鉴定
Zahra Hosseinali1, Jafar Mohammadshahi2, Amir Teimourpour3
1Department of Microbiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
Molecular biology reports
|November 16, 2023
概括
结核菌菌的耐药性是一个主要的公共卫生问题. 这项研究发现,与Mycobacterium tuberculosis complex (MTC) 菌株相比,非结核性真菌菌体 (NTM) 中的异化和利法抗性更高.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 公共卫生 公共卫生
背景情况:
- 伊索尼亚齐德和里法是治疗Mycobacterium结核病的重要一线药物.
- 耐药性对全球公共卫生构成重大威胁.
- 对抗性的分子检测对于有效的治疗策略至关重要.
研究的目的:
- 调查对异化和利法的药物耐药性的流行情况.
- 为了区分Mycobacterium结核复合体 (MTC) 和非结核菌 (NTM) 之间的耐药性模式.
- 采用MAS-PCR方法用于分子检测抗性.
主要方法:
- 一项涉及111个临床样本 (唾液,BAL) 的描述性横截面研究.
- 使用沸方法提取DNA.
- 特定序列-聚合酶链反应 (MAS-PCR) 的多重放大用于抵抗检测.
主要成果:
- 非结核性真菌菌 (NTM) 占分离物的15.3%.
- 总体抗药率为17%的异化和27%的利法.
- 在NTM菌株中,耐药性率显著更高:对异化的耐药性率为61.54%,对利法的耐药性为38.46%.
结论:
- 该研究强调了MTC和NTM中异化和利法抗性的流行情况.
- 与MTC相比,非结核性菌根菌表现出明显更高的抗结核药物耐药率.
- MAS-PCR是一种有效的方法,用于分子检测菌根菌感染中的耐药性.
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