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对ZmRL6积极调节玉米干旱应激耐受性的分子机制分析

Pengyu Zhang^1,2, Tongchao Wang¹, Liru Cao^1,2

¹National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, 450046, China.

|November 16, 2023

概括

玉米MYB相关基因ZmRL6增强了对干旱的耐受性. 过度表达提高了弹性,而淘汰赛增加了灵敏度,揭示了ZmRL6.

科学领域:

植物生物学植物生物学
分子遗传学分子遗传学
农业科学农业科学

背景情况:

与MYB相关的基因对植物生长,发育和应激反应至关重要.
与MYB相关的基因在玉米干旱压力中的特定作用在很大程度上是未知的.

研究的目的:

研究1R-MYB基因ZmRL6在玉米干旱应激反应中的功能.
识别ZmRL6向基因并阐明其调节网络.

主要方法:

基因克隆和ZmRL6.6的表征
在玉米中过度表达和CRISPR-Cas9敲除ZmRL6.
干旱压力测试.
DAP-seq和RNA-seq用于目标基因鉴定.

主要成果:

ZmRL6是一种高度干旱诱导的基因,在谷物中保存.
过度表达ZmRL6可以增强干旱耐受性.
ZmRL6淘汰突变体表现出增加的干旱过敏性.
ZmRL6直接向参与激素信号传递,糖代谢,素合成和氧化还原信号传递的基因.

结论:

ZmRL6在调节玉米干旱耐受性方面发挥着至关重要的作用.
ZmRL6作为玉米干旱反应途径的积极调节者.

关键词:

一个R-MYB.干旱造成的压力是干旱.与MYB相关的基因玉米玉米玉米是一种在 ZmRL6 中使用.

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