脂质丰富减少营养素培养基改善牛胚胎细胞形成
Rolando Pasquariello1, Mingxiang Zhang2, Jason R Herrick3
1R Pasquariello, Colorado Center for Reproductive Medicine, Lone Tree, United States.
Reproduction & fertility
|November 16, 2023
概括
补充减少营养的牛胚胎培养基与富含脂质的BSA改善了胚胎细胞的发育. 这种脂质特征增强了脂质代谢和胚胎质量,这表明了体外生产的精细方法.
科学领域:
- 生殖生物学 生殖生物学
- 动物科学动物科学
- 生物化学 生物化学
背景情况:
- 优化胚胎培养基对于提高体外生产效率和胚胎生存能力至关重要.
- 以前的研究表明,标准培养基含有过多的营养素,而营养环境减少可以支持有能力的胚胎发育.
研究的目的:
- 评估外源性脂质和L-卡尼丁补充剂对牛胚胎细胞发育的影响.
- 为了进一步完善牛胚胎的营养减少 (RN) 培养条件.
主要方法:
- 在对照介质 (100%的营养) 和RN介质 (6.25%的营养) 中培养牛肉,其中含有L-卡尼丁和无脂或富脂的牛血清白蛋白 (BSA).
- 分析了母细胞细胞的发育,细胞数,基因表达 (脂质/葡萄糖代谢,氧化还原平衡,胚胎质量),线粒体DNA拷贝数,ATP生产和脂质资料.
主要成果:
- 与控制和没有脂质的RN介质相比,与富含脂质的BSA减少的营养介质显示出胚胎细胞的发育增加.
- 然而,在两组RN组中,胚胎细胞数量都比对照组低.
- 来自脂质丰富的RN介质的胚胎表现出与脂质代谢,胚胎质量和氧化还原平衡相关的上调基因,与低调葡萄糖代谢基因.
结论:
- 富含脂质的BSA,富含特定的脂肪酸 (林诺基酸,牛酸,油脂酸,棕酸,α-林诺基酸),在营养减少的条件下支持牛胚胎的增强脂质代谢和胚胎细胞发育.
- 这表明,通过仔细补充减少的营养介质,可以优化体外胚胎的生产.
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