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在基基蛋白足迹实验中使用二甲基尿素作为消火剂

Anter A Shami1, Sandeep K Misra1, Lisa M Jones2

  • 1Department of BioMolecular Sciences, University of Mississippi, Oxford, Mississippi 38677, United States.

Journal of the American Society for Mass Spectrometry
|November 16, 2023
PubMed
概括

甲基尿素 (DMTU) 在不添加背景蛋白的情况下有效地杀基蛋白足迹 (HRPF) 反应. 这提高了复杂样本中的蛋白质识别,使得DMTU成为推的酶替代品.

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In vitro Mutagenesis01:16

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Crossed Aldol Reaction Using Weak Bases

This lesson deals with the crossed aldol reaction using weak bases. The self-condensation of an aldehyde having α hydrogen is prevented by adding it slowly to a mixture of formaldehyde and weak bases like hydroxide and alkoxide. Upon slow addition of the aldehyde, the base deprotonates the α carbon of the aldehyde to form the corresponding enolate. The enolate subsequently attacks the formaldehyde to form a single crossed product. Figure 1 depicts the aforementioned reaction.