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Updated: Jul 11, 2025

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

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一种代理组-RNA捕获方法揭示了处理体抑制正调节的枢纽基因

Chen Liu1,2, Andriani Mentzelopoulou1,3, Ioannis H Hatzianestis1,3

  • 1Department of Biology, University of Crete, Heraklion 70013, Greece.

The Plant cell
|November 16, 2023
PubMed
概括

研究人员开发了一种新的方法来识别细胞凝聚物中称为处理体 (PBs) 的RNA. 这项研究揭示了PB中的RNA组成和命运是如何动态调节的,影响植物应激反应.

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In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
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Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
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