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基于NMR的品味聚合物相互作用研究以及红酒对味觉感知的影响
Anna Maria Gabler1, Annalena Ludwig1, Oliver Frank1
1Chair of Food Chemistry and Molecular Sensory Science, TUM School of Life Sciences, Technical University of Munich, Lise-Meitner-Str. 34, D-85354 Freising, Germany.
Journal of agricultural and food chemistry
|November 16, 2023
概括
红酒中的味道化合物和聚合物之间的分子相互作用使用定量1H NMR进行了研究. 这些相互作用显著影响红葡萄酒.
科学领域:
- 食品化学 食品化学
- 葡萄酒化学 葡萄酒化学
- 分子相互作用 分子相互作用
背景情况:
- 了解红葡萄酒中的分子相互作用对于风味特征至关重要.
- 高分子量 (HMW) 聚合物显著影响葡萄酒的味道和口感.
- 之前的研究缺乏直接的分子洞察力,了解葡萄酒中的口味-聚合物相互作用.
研究的目的:
- 直接研究红酒中味觉活性化合物和HMW聚合物之间的分子相互作用.
- 通过使用先进的分析技术,建立Primitivo红葡萄酒的味道复制模型.
- 阐明这些相互作用对味觉和聚合物特性的影响.
主要方法:
- 定量1HNMR光谱分析分子相互作用随时间的推移.
- 液体染色学-并联质谱学 (LC-MS/MS) 用于化合物识别.
- 高性能离子色谱 (HPIC) 用于离子化合物分析.
- 用味道复制模型和不同的HMW分数进行感官实验.
主要成果:
- 根据1H NMR化学转移变化,确定了三个不同的相互作用场景.
- 弗拉万-3-和酸乙烯 Ester 显示向较低频率的转移,表明相互作用.
- 酸,有机酸,盐和醇转移到更高的频率.
- 高值分数 (30-50 kDa和>50 kDa) 呈现出显著的相互作用效应.
- 在pH值4.0时观察到最大的相互作用.
结论:
- 品尝剂和HMW聚合物之间的分子相互作用直接影响红葡萄酒的酸味和味的味道感知.
- 这些相互作用也会影响HMW聚合物的固有苦和性质.
- 这项研究为预测红葡萄酒的风味变化提供了分子基础,基于品味-聚合物相互作用.
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