在Caulobacter和φCbK中保存的XRE转录因子调节粘合素的发育和菌体的产生
Maeve McLaughlin1, Aretha Fiebig1, Sean Crosson1
1Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, United States of America.
PLoS genetics
|November 16, 2023
概括
在Caulobacter和它的菌体中,共享的异生生物反应元素 (XRE) 转录因子 (TF) 的基因群影响细菌粘附并控制菌体感染动态. 这些XRETFs调节宿主基因,这些基因参与了固的发育和菌体的产生.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 异生物反应元 (XRE) 转录因子 (TFs) 在细菌和菌体中普遍存在,调节细胞生理和菌体与宿主相互作用.
- 在Caulobacter物种中,类似的XRE TFs的基因集群已知在坚固粘附蛋白的发展和生物膜形成中的作用.
研究的目的:
- 调查Caulobacter crescentus和Caulophage φCbK之间共享的XRE TFs的作用.
- 了解这些XRETFs如何影响宿主-菌体相互作用,包括粘附和病毒生成.
主要方法:
- 对Caulobacter物种的泛基组分析,以确定XRE TF基因集群.
- 对XRE TF相互作用和对常见基因组的转录控制的实验分析.
- 在C. crescentus中进行基因删除和过度表达研究,以评估对φCbK菌体爆发大小的影响.
主要成果:
- 来自C. crescentus和φCbK的一组XRETFs在物理上相互作用,并共同调节参与holdfast发育和φCbK病毒生成的基因.
- 编码于φCbK的XRE TF,tgrL,在感染过程中表达高,并抑制宿主基因,如hfiA (持久性抑制剂) 和gafYZ (基因转移剂激活剂).
- 主体XRETFs也抑制了gafyz,这表明它对伪基因转移剂的产生和溶解有保护机制.
- 删除C. crescentus XRE TFs减少了φCbK爆发大小,通过过度表达宿主XREs或φCbK tgrL来挽救.
结论:
- 在C. crescentus和φCbK共享的XRE TF基因集群对于在非传染条件下调节粘附至关重要.
- 这些XRETF在感染期间调节宿主-菌体动态方面发挥着重要作用,影响菌体产量.
- 这些发现揭示了由XRE转录因子调解的宿主-菌体相互作用的保存机制.
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