在成年人患有酸性食道炎时,对局部食品注射的急性粘膜反应的转录形状分析
Mirelle T A Kleuskens1, Maria L Haasnoot2, Johan Garssen3
1Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, Utrecht, The Netherlands.
The Journal of allergy and clinical immunology
|November 16, 2023
概括
性食道炎 (EOE) 患者在暴露于食物过敏原后,食道中呈现迅速的基因表达变化. 在EoE中,TNFSF18 (GITRL) 被确定为早期免疫反应对食物过敏原的关键分子.
科学领域:
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
- 分子生物学分子生物学
背景情况:
- 性食道炎 (EOE) 涉及急性食道粘膜对食物过敏原的反应.
- 在EoE中驱动这些急性反应的局部免疫机制尚未完全理解.
研究的目的:
- 在EoE患者的急性食物过敏原挑战期间,研究食道粘膜中早期的转录基因变化.
主要方法:
- 从EoE患者 (n=5) 和对照组 (n=5) 的食道活检中大量RNA测序.
- 在EoE患者注射内食物提取物之前和20分钟后采集样本.
主要成果:
- 在EoE患者中,EoE基因签名的基线呈现较高.
- 包括CEBPB,IL1B,TNFSF18,PHLDA2和SLC15A3在内的40个基因被确定为早期免疫反应的关键.
- TNFSF18 (GITRL) 显示显著增加,并被IL-13和TNF-α迅速诱导到食道上皮细胞中.
结论:
- 这项研究揭示了转录组变化,在EoE中调解了急性食物过敏原反应.
- TNFSF18 (GITRL) 被认为是EoE对食物过敏原反应早期的关键效应分子.
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