单细胞蛋白和甲的微生物电合成,通过将快速生长的 Methanococcus maripaludis 与微生物电解细胞相结合
1Department of Biological and Ecological Engineering, Oregon State University, Corvallis, OR 97331, United States; Department of Biological Engineering, Utah State University, Logan, UT 84322, United States.
Bioresource technology
|November 16, 2023
概括
通过使用微生物电解细胞 (MEC) 与Methanococcus maripaludis结合,增强单细胞蛋白 (SCP) 的产生. 这种方法提供了一种可持续的替代蛋白质来源,具有高蛋白质含量和必需氨基酸.
科学领域:
- 生物技术是生物技术.
- 微生物学 微生物学
- 可持续农业 可持续农业
背景情况:
- 工业农业面临着传统动物蛋白质生产的挑战.
- 单细胞蛋白 (SCP) 提供了一个可持续的替代蛋白质来源.
- 微生物电解细胞 (MEC) 可以与微生物结合用于生物生产.
研究的目的:
- 调查使用快速生长的甲原体Methanococcus maripaludis用于SCP生产的潜力.
- 评估微生物电解细胞 (MEC) 在支持SCP合成方面的效率.
- 描述产生的SCP的蛋白质含量和氨基酸概况.
主要方法:
- 在微生物电解细胞 (MECs) 中培养甲菌maripaludis.
- 监测细胞生长,甲生产和SCP产量.
- 对收获生物质的蛋白质含量和氨基酸成分的分析.
- 电子流量分析以确定用于SCP合成的电子利用效率.
主要成果:
- 在MECs中,Methanococcus maripaludis的翻倍时间为11.2小时,特异性增长率为0.062 1/h.
- 取得的最高SCP生产率为13.7 mg/L/h.
- 干燥的生物质含有60%以上的蛋白质,富含必需氨基酸.
- 在MEC中的31.3%的电子被指向SCP合成.
结论:
- 将快速生长的甲基生物与MEC相结合是有效生产SCP的可行策略.
- 这种方法为传统蛋白质来源提供了一个可持续的替代方案.
- 生产的SCP富含蛋白质,具有有利的氨基酸特征,适合各种应用.
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