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双胞胎-Jejunal绕道改善新陈代谢和通过调节ceRNA网络重新模拟细胞外基质

Qingnan Zhao¹, Jiaxin Cheng², Aimei Gao³

¹Department of Clinical Pharmacy, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200020, China; Shanghai Key Laboratory of Pancreatic Disease, Shanghai Jiao Tong University School of Medicine, Shanghai 201620, China.

|November 16, 2023

概括

双胞胎关突围 (DJB) 手术改变脂肪组织中的非编码RNA表达,通过通过ceRNA网络重塑细胞外基质 (ECM) 影响脂质代谢和胰岛素抵抗.

科学领域:

代谢手术代谢手术
肥胖问题研究研究
在RNA生物学,RNA生物学.

背景情况:

腹腔外科手术 (BS),包括十二指肠-关节绕道 (DJB),有效治疗肥胖和2型糖尿病 (T2DM).
以前的研究表明,DJB会改变胃肠道长非编码RNA (lncRNAs),通过肠-大脑和肠-胰腺轴影响脂质代谢和血糖控制.
DJB对脂肪组织非编码RNA表达特征和竞争性内源RNA (ceRNA) 网络的影响需要进一步调查.

研究的目的:

为了比较lncRNA,圆形RNA (circRNA) 和信使RNA (mRNA) 在DJB后脂肪组织中的表达.
为了研究ceRNA调节网络受DJB在脂肪组织中的影响.
阐明DJB对新陈代谢和胰岛素敏感性的影响背后的分子机制.

主要方法:

在模拟和DJB组的脂肪组织中对lncRNAs,circRNAs和mRNAs的差异表达分析.
基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 对差异表达mRNA (DEmRNA) 的途径分析.
构建和分析涉及DElncRNAs,DEcircRNAs和DEmRNAs的ceRNA调节网络.

主要成果:

确定了2219个DEmRNA,722个DElncRNA和425个DEcircRNA.
德米RNA与脂质代谢,氨基酸代谢,胰岛素抵抗和细胞外基质 (ECM) 途径有关.
分析揭示了ceRNA网络中的31个枢纽DE mRNA,包括Mpp7,9330159F19Rik,Trhde,Trdn和Sorbs2.

结论:

DJB影响脂肪组织重塑,特别是ECM.
观察到的代谢改善是通过ceRNA调节网络进行的.
DJB对能量代谢的影响与改变的非编码RNA表达和随后的途径调制有关.

关键词:

脂肪组织的脂肪组织.十二指管jejunal 绕道进行ECM重建.改善新陈代谢的改善网络 ceRNA 网络

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