聚醇,抗氧化剂和Lecythis pisonis的伤口愈合 种子外套
Elisângela Flávia Pimentel1, Bruno Gomes de Oliveira2, Ana Claudia Hertel Pereira1
1Pharmaceutical Science Graduate Program, Vila Velha University, Vila Velha, ES, Brazil.
Planta medica
|November 16, 2023
概括
沙普卡亚种子外套表现出强大的抗氧化剂和伤口愈合特性,没有细胞毒性. 这种提取物促进纤维细胞增殖,刺激生长因子,显示了皮肤学化品应用的潜力.
科学领域:
- 药理学 药理学是指药理学的学科.
- 生物化学 生物化学
- 皮肤病学 皮肤病学
背景情况:
- 莱西蒂斯·皮索尼斯生物量,特别是萨普卡亚种子外层,未得到充分利用.
- 研究伤口愈合剂的自然来源对于开发新型治疗和化品至关重要.
研究的目的:
- 为了评估Sapucaia种子外套提取物的伤口愈合潜力.
- 分析其抗菌,抗氧化和细胞毒性作用.
- 确定其刺激血管内皮生长因子-A (VEGF-A) 和血小板衍生生长因子 (PDGF) 的能力.
主要方法:
- 使用电子喷雾电离的化学成分分析 里埃转换离子 循环电共振质谱学.
- 通过DPPH激素清除和铁减少抗氧化功率测试来评估抗氧化活性.
- 在体外对细胞毒性,纤维细胞增殖和生长因子刺激的评估.
- 使用最小抑制度 (MIC) 确定对黄金葡萄球菌的抗菌活性.
主要成果:
- 鉴定出了19种化合物,包括益西亚尼丁和黄类化合物,它们有助于抗氧化活性.
- 提取物显示出强大的抗氧化强度 (IC50 = 0.99μg/mL对于DPPH) 和铁的降解能力 (IC50 = 1.09μg/mL).
- 没有观察到细胞毒性;证实了显著的纤维细胞迁移/增殖 (p<0.05) 和剂量依赖的VEGF-A和PDGF刺激.
- 观察到对黄金葡萄球菌的抗菌活性,MIC为31.25μg/mL.
结论:
- 沙普卡亚种子外套提取物具有显著的抗氧化,抗菌和促进伤口愈合的特性.
- 提取物刺激参与组织修复的关键生长因子,促进纤维细胞活性.
- 这些发现支持Sapucaia种子外套提取物在皮肤化品中用于伤口愈合的潜在用途.
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