人类微生物组的系统挖掘识别了具有多样化活性光谱的抗菌
Andrew M King1, Zhengan Zhang1, Emerson Glassey1
1Synthetic Biology Center, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Nature microbiology
|November 17, 2023
概括
人类细菌产生修饰的,影响宿主健康和微生物群落. 研究人员从这些中发现了新型抗生素,这些抗生素对各种病原体和导致失生症的菌株有效.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 基因组学就是基因组学.
背景情况:
- 人类相关的细菌产生改性来调节宿主生理和微生物组成.
- 在这些相互作用中,核糖体合成和翻译后修饰 (RiPPs) 是至关重要的.
研究的目的:
- 为了识别和描述人类相关细菌的RiPPs.
- 从这些RiPP中发现新的抗菌化合物.
主要方法:
- 基因组挖掘2,229个人类微生物组项目细菌物种的RiPP基因集群.
- 在大肠杆菌中发现的RiPPs的合成和表达.
- 精选的RiPPs的净化和功能特征.
- 对人类植物和病原性细菌进行抗微生物活性测试.
主要成果:
- 鉴定了218种兰西和25种拉索.
- 成功合成和表达了70个RiPP,其中23个被净化和特征化.
- 发现了新的抗生素,针对与皮肤,鼻腔和阴道失调相关的细菌.
- 发现扩展和狭窄谱抗生素对抗抗甲素耐药的金黄色葡萄球菌和抗万科胺耐药的菌菌有效.
结论:
- 人类相关的微生物是新型抗菌化合物的丰富来源.
- 从人类微生物群中挖掘RiPP可以揭示生态关系,并产生新的候选药物.
- 这项研究强调了RiPPs在打击抗生素耐药性的潜力.
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