基于CRISPR的光热纳米:各种生物纳米粒子操纵和单核酸识别
Jiajie Chen1, Zhi Chen2, Changle Meng2
1State Key Laboratory of Radio Frequency Heterogeneous Integration, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronics Engineering, Shenzhen University, Shenzhen, 518060, China. cjj@szu.edu.cn.
Light, science & applications
|November 17, 2023
概括
采用CRISPR驱动的光热纳米 (CRONT) 能够同时操纵和识别生物纳米粒子. 这项创新增强了单分子DNA检测和分离观测,用于护理点诊断.
科学领域:
- 生物物理学的生物物理.
- 纳米技术纳米技术
- 分子生物学分子生物学
背景情况:
- 光热纳米器提供先进的纳米粒子操纵.
- 目前的应用主要集中在操纵上,在生物纳米粒子识别中使用有限.
- 光热场对生物检测的潜力在很大程度上仍未被探索.
研究的目的:
- 通过将光热操纵与基于CRISPR的生物检测相结合,开发CRISPR驱动的光热纳米 (CRONT).
- 为了利用扩散泳和热透流来捕获和丰富各种生物纳米粒子.
- 建立一个光热方案,用于增强单核酸多态 (SNP) 的单分子检测,并观察核酸裂变.
主要方法:
- 将光热纳米与CRISPR-Cas系统集成在一起.
- 利用光热激发来产生扩散泳和热奥斯摩斯流.
- 金纳米粒子与DNA的功能化,用于有针对性的捕获和丰富.
- 开发新的CRISPR方法来增强分子事件的检测和观察.
主要成果:
- 成功地捕获和丰富了DNA功能化的黄金纳米粒子,CRISPR相关的蛋白质和DNA链.
- 一个光热方案的演示显著提高基于CRISPR的单核酸多态 (SNP) 在单个分子水平的检测.
- 介绍了一种新的CRISPR方法来观察核酸裂变事件.
- 通过使用光学笔,在水溶液中实现了DNA识别能力.
结论:
- 克朗特代表了一项重大进步,使生物纳米颗粒能够同时在现场进行操纵和识别.
- 该技术提供了高的特异性和可行性,为先进的诊断铺平了道路.
- 克朗特准备成为医疗诊断,生物光子学和生物纳米技术中的通用工具.
相关概念视频
01:59CRISPR
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
02:31Homologous Recombination
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02:53CRISPR and crRNAs
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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01:28CRISPR/Cas9 Genome Editing
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...