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Updated: Jul 11, 2025

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使用高斯混合模型提高单粒子冷EM结构的分辨率和可分辨性

Muyuan Chen1, Michael F Schmid2, Wah Chiu2,3

  • 1Division of CryoEM and Bioimaging, SSRL, SLAC National Accelerator Laboratory, Stanford University, Menlo Park, CA, USA. muyuanc@stanford.edu.

Nature methods
|November 17, 2023
PubMed
概括

这项研究引入了一种新的高斯混合模型协议,通过更好地调整灵活的蛋白质结构来改善冷电磁波分辨率. 该方法通过完善宏分子动力学来增强结构生物学见解.

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Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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