肥胖诱导的Ly6C高和Ly6C低单细胞子群的变化取消了中风恢复中风后缺血四肢调节的好处
Il-Doo Kim1, Hyunwoo Ju1, Joseph Minkler1
1Burke Neurological Institute, White Plains, NY, USA.
概括
远程肢体调节 (RLC) 通过改变单细胞来改善中风的结果. 然而,肥胖症阻止了这种有益的单细胞转移,否定了RLC.
科学领域:
- 神经科学是一个神经科学.
- 免疫学 免疫学 免疫学
- 血管生物学 血管生物学
背景情况:
- 远程肢体调节 (RLC) 是一种涉及间歇性肢体血流中断的技术.
- RLC激活了内源性保护机制,改善了中风的结果.
- 保护作用与循环单细胞转移到促炎性Ly6C高子集有关.
研究的目的:
- 调查肥胖对RLC中风疗效的影响.
- 为了确定RLC是否仍然可以在肥胖中风模型中提供好处.
主要方法:
- 瘦和肥胖小鼠中风结果的比较.
- 评估循环单细胞形状和脑免疫细胞透情况.
- 评估RLC对肥胖和瘦小鼠单细胞子集的影响.
- 在体外分析使用从瘦和肥胖小鼠的RLC血清化的胞.
主要成果:
- 与瘦小鼠相比,肥胖的中风小鼠表现出更高的单细胞化,增加的单细胞/巨细胞透,更差的结果和延迟的恢复.
- 肥胖小鼠的RLC治疗没有诱导在瘦小鼠中观察到的有益单细胞转移.
- 在肥胖小鼠中没有单细胞转移,这就取消了RLC的功能益处.
- 肥胖小鼠的RLC血清未能在体外诱导单细胞转移,而不是瘦小鼠的血清.
结论:
- 肥胖会改变单细胞和单细胞子集的特征,阻碍中风中RLC的保护作用.
- 在肥胖个体中,无法将单细胞转移到促炎状态,从而抵消了RLC的治疗效益.
- 像肥胖等并发症在将RLC作为中风治疗时需要仔细考虑.
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