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相关概念视频

Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
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Updated: Jul 11, 2025

Isolation and Transcriptome Analysis of Plant Cell Types
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单细胞转录组图谱和发展棉花的监管动力学

Yanlong Li1, Huanhuan Ma1, Yuanlong Wu1

  • 1National Key Laboratory of Crop Genetic Improvement & Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|November 17, 2023
PubMed
概括

高温会通过扰乱其他植物的发育,导致植物的雄性不育. 这项研究揭示了热应激如何影响特定的细胞,为创造耐热作物提供了洞察力.

关键词:
其他类型的.高温的高温的高温的高温单细胞RNA测序 (scRNA-seq) 是一种一个单细胞多细胞的奥米克.这是一张 tapetum tapetum.

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Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
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科学领域:

  • 植物生殖生物学 植物生殖生物学
  • 分子遗传学 分子遗传学
  • 农业科学 农业科学

背景情况:

  • 植物体含有专门的细胞,对于繁殖至关重要.
  • 高温 (HT) 压力会导致男性不育,降低作物产量.
  • 在细胞层面上理解HT的发育和HT反应是有限的.

研究的目的:

  • 创建第一个单细胞转录组图谱和棉花的染色质可访问性调查.
  • 在细胞发育过程中绘制细胞特异性基因表达和表观遗传景观的地图.
  • 研究HT应激对其他细胞功能的影响,并确定关键的调节机制.

主要方法:

  • 单细胞RNA测序 (scRNA-seq) 用于转录组分析.
  • 用测序 (ATAC-seq) 检测转化酶可访问的染色质.
  • 细胞发育轨迹的生物信息重建.

主要成果:

  • 棉花细胞的详细空间表达图谱和表观遗传景观.
  • 识别新型基因和精确的发育时间介质, tapetal 和中间层细胞.
  • 对于花粉壁合成至关重要的带状细胞对HT压力最敏感.
  • 通过降低染色质可访问性,HT压力会使面膜细胞中的关键基因 (如QRT3,CYP703A2) 沉默,从而导致异常花粉和男性不育.

结论:

  • 这项研究提供了关于棉花的发展和细胞对热量的反应的全面资源.
  • 它阐明了HT诱导的男性不孕症背后的分子机制.
  • 这些发现为培育耐热作物提供了潜在的策略.