端粒酶表达与埃及HCV患者的PBMC对HCV核心抗原的免疫反应不佳有关
Iman H Ibrahim1, Ola Sayed M Ali1, Adel A El-Sahar2
1Department of Biochemistry and Molecular Biology, Faculty of Pharmacy (Girls), Al-Azhar University, Egypt.
Journal of clinical and experimental hepatology
|November 17, 2023
概括
血清人类端粒酶 (hTERT) 水平随着肝病的进展而增加. 肝细胞癌 (HCC) 患者的T细胞反应降低和记忆细胞枯竭表明hTERT和HCC发展之间存在联系.
科学领域:
- 肝病学和免疫学 肝病学和免疫学
- 在瘤学瘤学.
背景情况:
- 肝细胞癌 (HCC) 是最常见的原发性恶性肝脏瘤.
- 了解推动HCC进展的分子机制对于有效的治疗策略至关重要.
研究的目的:
- 为了研究与肝病进展有关的血清人类端粒酶 (hTERT) 水平.
- 评估型肝炎病毒 (HCV) 核心蛋白对具有或没有HCC的HCV患者内存T细胞的影响.
- 为了将记忆T细胞表型与HCV患者的疾病进展相关联.
主要方法:
- 使用相对定量的RT-PCR量化血清hTERT水平.
- 通过流式细胞计量评估T细胞反应 (IFN-γ分泌) 和记忆CD8+细胞表型.
- 用HCV核心蛋白刺激T细胞以评估功能变化.
主要成果:
- hTERT水平从对照组逐步增加到慢性肝炎,肝硬化和HCC患者.
- 与对照组和非HCC HCV患者相比,T细胞响应 (IFN-γ分泌) 在HCC患者中呈现逐步下降.
- 晚分化的记忆CD8+T细胞 (CD8+,CD27-,CD28-,CD45RA+,CCR7-) 在HCC患者中显著耗尽.
结论:
- 在HCV患者中,hTERT水平和T细胞IFN-γ分泌之间存在负相关性.
- 升高的hTERT,降低的T细胞免疫力和记忆细胞枯竭之间的相互作用可能会导致肝病进展为HCC.
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