在Bixa orellana中的annatto色素 (bixin) 生物合成的植物年龄依赖的动态
Kleiton Lima de Godoy Machado1, Daniele Vidal Faria1, Marcos Bruno Silva Duarte1
1Departamento de Biologia Vegetal/Laboratório de Cultura de Tecidos Vegetais/BIOAGRO, Campus Universitário, Universidade Federal de Viçosa, 36570-900, Viçosa, MG, Brazil.
Journal of experimental botany
|November 17, 2023
概括
改变秋叶树中的microRNA156 (miR156) 含量会影响叶子的发育,并通过影响miR156-SPL模块来降低白产量,从而提供新的育种策略.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 代谢工程是代谢工程.
背景情况:
- 植物的二次代谢物产量取决于年龄,但调节机制尚不清楚.
- 树 (Bixa orellana) 生产了宝贵的阿波卡洛类药物 - - ,其生物合成调节尚不清楚.
- 微RNA156 (miR156) - SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) 途径是植物发育的保守调节者.
研究的目的:
- 调查miR156-SPL模块在Bixa orellana叶子中调节年龄相关的素生物合成中的作用.
- 了解操纵miR156如何影响比克辛的产生和相关的代谢途径.
- 探索该模块在优化 bixin 产量的潜在应用.
主要方法:
- 在Bixa orellana (miR156ox) 中过度表达miR156,以研究其对基因表达和代谢物产生的影响.
- 分析BoSPL转录水平,叶子发育,比克辛含量和酸水平.
- 关键胡卜素生物合成基因 (BoCCD4-4,BoCCD1) 的基因表达分析和二次代谢物酶的蛋白质组分析.
主要成果:
- miR156的过度表达降低了BoSPL转录水平,改变了叶子的发育.
- 在miR156ox植物中,双素的产量减少,而酸水平增加.
- 碳流从素转移到酸,关键的二次代谢酶的积累较低.
结论:
- 保存的BomiR156-BoSPL模块调节了秋树的叶子发育和素生物合成.
- 该模块影响碳流向酸生产,并影响二次代谢.
- 结果为操纵B. orellana繁殖计划中的 bixin生产提供了洞察力.
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