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牛性口腔炎病毒作为牛的疫苗载体

Gustavo Delhon¹, Sushil Khatiwada^2,3, David Doub²

¹School of Veterinary Medicine & Biomedical Sciences, University of Nebraska, Lincoln, Nebraska, USA.

The Journal of general virology

|November 17, 2023

概括

牛性口腔炎病毒 (BPSV) 显示出作为牛疫苗的新型病毒载体的希望. 再组合的BPSV表达关键的病毒糖蛋白,成功诱导了牛群免疫力和对牛疹病毒1 (BoHV-1) 感染的保护.

科学领域:

兽医病毒学兽医病毒学
疫苗开发疫苗开发
分子生物学分子生物学

背景情况:

目前缺乏商业上可用的病毒载体疫苗用于牛.
牛性口腔炎病毒 (BPSV) 是一种在牛中普遍存在的帕洛克斯病毒,具有适合病毒载体的特征.
这些特征包括它对外来DNA插入的能力,诱导轻度抗病毒免疫力,以及牛群中的持久性.

研究的目的:

评估BPSV作为病毒载体在牛群中开发疫苗的潜力.
为了构建表达牛疹病毒1 (BoHV-1) 的复合BPSV糖蛋白.

主要方法:

重组BPSV菌株,BPSV^gD和BPSV^gD/gB,被设计成表达BoHV-1糖蛋白gD和gB.
血清阳性BPSV小牛用复合BPSV构造物进行免疫.
接种疫苗的小牛随后被高剂量的BoHV-1挑战.

主要成果:

用BPSV^gD或BPSV^gD/gB进行免疫接种成功诱导了牛中的BoHV-1中和抗体.
免疫接种的四只小牛中有三只在免疫接种后70天内对高剂量的BoHV-1挑战进行了保护.
该研究证明了复合BPSV在引起保护性免疫反应方面的有效性.

结论:

牛性口腔炎病毒 (BPSV) 是一个合适的候选人,可以作为牛疫苗中的病毒载体开发.
使用BPSV作为载体为控制BoHV-1等具有经济意义的牛病提供了潜在的新策略.
对基于BPSV的疫苗的进一步研究可能会导致牛的新型疾病预防方法.

关键词:

牛性疹病毒 1 牛性疹病毒牛乳腺口腔炎病毒 (Bovine Papular Stomatitis Virus) 已经成为一种常见疾病.挑战挑战挑战挑战免疫接种免疫接种免疫接种病毒载体病毒载体

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