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Updated: Jul 11, 2025

Author Spotlight: Two-Step Tag-Free Isolation of Mitochondria for Improved Protein Discovery and Quantification
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协议研究人类线粒体核糖体使用量化密度梯度分析通过质谱学和复合体分析分析分析分析.

Petra Páleníková1, Michal Minczuk1, Pedro Rebelo-Guiomar1

  • 1MRC Mitochondrial Biology Unit, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK.

STAR protocols
|November 17, 2023
PubMed
概括

这项研究引入了一种新的方法来分析大型蛋白质复合体,如人类线粒体核糖体,在接近本地条件下. 这种方法将密度梯度超离心与质谱相结合,用于详细的分子复杂分析.

关键词:
生物信息学是一种生物信息学.蛋白质表达和净化过程蛋白质组学是指蛋白质组学.

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Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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