在操作环境中对持续UV-A照明进行临床前安全评估
Rachael Guenter1, Rui Zheng-Pywell1, Brendon Herring1
1Department of Surgery, University of Alabama at Birmingham, Birmingham, Alabama, United States of America.
PloS one
|November 17, 2023
概括
新的UV-A光消毒对手术室来说是安全的,显示的DNA损伤最小,对工作人员来说是安全的暴露水平. 这在手术期间提供了增强的抗微生物保护,以减少手术部位感染 (SSI).
科学领域:
- 微生物学 微生物学
- 生物物理学的生物物理.
- 手术创新 在外科创新.
背景情况:
- 杀菌性紫外线C (UV-C) 光对消毒有效,但会导致DNA损伤,限制其在手术内使用.
- 手术部位感染 (SSIs) 影响5-15%的患者,增加病率和成本.
- 需要持续的手术内消毒,以最小的DNA损伤风险.
研究的目的:
- 为了评估手术内紫外线A (UV-A) 光用于消毒的安全性.
- 评估医务人员在UV-A暴露期间的DNA损伤和反射水平.
- 为了比较UV-A和UV-C光对DNA损伤和修复的影响.
主要方法:
- 在体外研究中,研究了UV-A和UV-C对DNA损伤和修复的影响.
- 小鼠模型评估了来自UV-A和UV-C的DNA损伤光生成.
- 猪类临床前研究评估了外科人员和个人防护设备的紫外线辐射.
主要成果:
- 紫外线A光在细胞或组织层面上没有显著的DNA损伤.
- 猪模型中的外科人员暴露于安全的紫外线A辐射水平.
- 通过手术个人防护设备 (PPE) 传播的紫外线仍在安全范围内.
结论:
- 紫外线 (UV-A) 光为持续的手术内消毒提供了一个潜在的安全选择.
- 这项技术可以通过减少SSI来提高患者的安全性.
- 进一步的研究可能会验证UV-A作为标准的手术内消毒工具.
相关概念视频
01:02Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview
Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material, molecules absorb light depending on the energy required for electronic transitions. As a result...
01:14UV–Vis Spectrometers
The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...