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Updated: Jul 11, 2025

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拦截近距离标记用于询问细胞外流微环境

Guyu Wang1, Qiang Li1, Yuna Guo1

  • 1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

Analytical chemistry
|November 17, 2023
PubMed
概括

研究人员开发了一个拦截近距离标记 (IPL) 平台,以跟踪细胞外过氧化 (H2O2) 流量. 这个工具可视化了氧化应激,并识别了参与细胞相互作用的蛋白质,推进了氧化还原信号研究.

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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