使用下一代DNA测序识别和分析脚微生物组:观察性分析
Caroline Hoch1, J Ryan Allen, Joshua Morningstar
1From the Department of Orthopaedics and Physical, Medical University of South Carolina, Charleston, SC (Allen, Morningstar, Materon, Scott, and Gross), and the University of North Carolina, Gillings School of Global Public Health, Chapel Hill, NC (Hoch).
The Journal of the American Academy of Orthopaedic Surgeons
|November 17, 2023
概括
下一代测序揭示了一些患者独特的脚微生物组,其中Cutibacterium acnes和Staphylococcus epidermidis是最常见的细菌. 细菌存在与患者的结果或并发症没有相关性.
科学领域:
- 整形外科手术 整形外科手术
- 微生物学 微生物学
- 基因组学就是基因组学.
背景情况:
- 下一代测序 (NGS) 增强了细菌检测灵敏度,有助于在骨科中进行微生物组研究.
- 了解关节的原生微生物群对于手术结果至关重要.
研究的目的:
- 为了确定人类脚组织中是否存在独特的微生物群.
- 调查脚微生物组组件对患者结果的影响.
- 确定皮肤微生物是否是土著脚微生物群的一部分.
主要方法:
- 对32名接受脚关节整形或关节切除的患者进行前性队列研究.
- 从五个脚层收集的抽样:皮肤,视网膜,小腿前,关节囊和远距离的小腿骨 (N=157).
- 使用NGS分析的样本;记录了患者的人口统计和手术结果.
主要成果:
- 19%的脚抽样显示细菌存在; 81%是负的.
- 最常见的细菌是Cutibacterium acnes (25%) 和Staphylococcus epidermidis (6.25%) 这两种细菌.
- 细菌最常见于视网膜 (29.6%);根据NGS结果,没有观察到并发症的显著差异.
结论:
- 一些患者存在独特的脚微生物组,与皮肤菌群不同.
- 杆菌和Staphylococcus epidermidis在脚微生物群中很常见.
- 脚组织中的细菌DNA残留与不良的外科手术结果没有相关.
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