相关实验视频

Updated: Jul 11, 2025

In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells

In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells

Published on: September 28, 2017

黑色素通过AMPK/mTOR通路调节自,促进绵羊的白色细胞中的孕激素分泌

Hongwei Duan¹, Shuai Yang¹, Longfei Xiao²

¹College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, 730070, Gansu, China; Gansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation, Lanzhou, 730070, Gansu, China.

|November 17, 2023

概括

黑色素增强了绵羊的孕激素分泌.

科学领域:

生殖生物学生殖生物学
内分泌学在内分泌学.
细胞生理学细胞生理学

背景情况:

黄体对孕激素的产生至关重要.
黑素会影响白细胞中的自和孕分泌.
黄体中黑激素,自和孕激素分泌之间的相互作用仍然不清楚.

研究的目的:

研究黑激素在调节羊体黄体通过自的孕激素分泌中的作用.
分析不同阶段在羊的白色体中对黑激素受体,自标记物和类固醇蛋白的表达.
阐明AMPK/mTOR通路和黑激素受体在黑激素诱导的孕激素分泌中的参与.

主要方法:

通过ELISA,免疫组织化学和西式涂抹,分析黑素受体 (MT1,MT2),自蛋白 (Beclin1,LC3B),孕激素,StAR和CYP11A1的表达.
在体外培养的质细胞治疗与黑色素,自抑制剂 (氨酸) 和黑色素受体抑制剂 (4--2-propionamidotetralin,luzindole).
使用抑制剂 (化合物C) 评估AMPK/mTOR途径,以确定其在黑激素对白细胞影响中的作用.

主要成果:

在中间阶段的红色体中观察到黑激素受体,自和孕激素的最高表达.
黑色素治疗在培养的白色细胞中促进了自和孕分泌.
黑素对自和孕激素分泌的影响通过其受体和AMPK/mTOR通路进行介导.

结论:

黑色素至少部分地通过通过AMPK/mTOR通路的受体介导自的调节,促进绵羊质细胞中的孕激素分泌.
这项研究首次证明了自在羊体黄体中黑激素介导的孕激素分泌中的作用.
这些发现为了解黑激素在绵羊卵巢功能中的作用提供了基础.

关键词:

自自是一种自的过程.红色体 (Corpus luteum) 是一个体.黑色素是什么黑色素是什么黑色素是什么孕激素 (Progesterone) 是一种激素.绵羊是一种羊.

更多相关视频

Instrumentation of Near-term Fetal Sheep for Multivariate Chronic Non-anesthetized Recordings

Instrumentation of Near-term Fetal Sheep for Multivariate Chronic Non-anesthetized Recordings

Published on: October 25, 2015

Author Spotlight: Automated Infusion and Blood Sampling for Precise Hormonal Analysis in Conscious Mice

Author Spotlight: Automated Infusion and Blood Sampling for Precise Hormonal Analysis in Conscious Mice

Published on: August 25, 2023

相关概念视频

Hormonal Regulation of the Menstrual Cycle

Hormonal Regulation of the Menstrual Cycle

The ovarian cycle regulates endometrial changes throughout a single menstrual cycle via the coordinated action of gonadotrophin-releasing hormone (GnRH) and gonadotrophins.
At puberty, GnRH begins a pulsatile release pattern, which triggers the anterior pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The frequency and amplitude of GnRH pulses vary across the menstrual cycle, with faster pulses favoring LH release and slower pulses favoring FSH...

Management of Insomnia

Management of Insomnia

The sleep cycle, an integral part of human health, consists of several stages with distinct characteristics and functions. It begins with a transition from wakefulness to sleep, known as the light sleep phase, followed by the restorative deep sleep phase, essential for physical recovery and growth. The cycle concludes with the Rapid Eye Movement (REM) phase, characterized by high brain activity and vivid dreaming. Insomnia, a prevalent sleep disorder, involves difficulty falling asleep, staying...

Circadian Rhythms and Gene Regulation

Circadian Rhythms and Gene Regulation

The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...

Ovarian Cycle

Ovarian Cycle

The menstrual cycle includes a critical component known as the ovarian cycle, which undergoes two main phases each month—the follicular phase and the luteal phase. The follicular phase is variable and averaging around 14 days. Ovulation, triggered by a surge in luteinizing hormone (LH), marks the transition between the two phases. The second phase, the luteal phase, is relatively consistent, lasting approximately 14 days, and is marked by the activity of the corpus luteum. While a cycle...

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...

mTOR Signaling and Cancer Progression

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...