相关实验视频

Updated: Jul 11, 2025

X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050

X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050

Published on: May 13, 2020

来自Pseudomonas syringae的MucD结构揭示了类似HtrA的血清蛋白酶的N端环介导的三元化

Ju Hyeong Kim¹, Gwan Hee Lee¹, Jae-Hee Jeong²

¹College of Pharmacy, Chung-Ang University, Seoul, 06974, Republic of Korea; Department of Global Innovative Drugs, Graduate School of Chung-Ang University, Seoul, 06974, Republic of Korea.

Biochemical and biophysical research communications

|November 17, 2023

概括

这项研究揭示了Pseudomonas syringae MucD (psMucD) 的结构,这是一个HtrA血清蛋白质酶. 它阐明了psMucD的情况.

科学领域:

生物化学生化学
结构生物学结构生物学
微生物学微生物学

背景情况:

蛋白质质量控制对细胞完整性至关重要,HtrA血清蛋白酶管理 prokaryotes 中的周等离子体折叠应激.
大肠杆菌有三个HtrA成员 (DegS,DegP,DegQ);MucD是一种涉及酸盐生物合成和应激反应的相关蛋白质.
假杆菌 (Pseudomonas syringae) 导致植物疾病和人类感染,需要了解其毒性因素,如MucD.

研究的目的:

为了确定来自Pseudomonas注射器 (psMucD) 的MucD的高分辨率结构.
描述psMucD的寡合状态及其三元化机制.
将psMucD的结构和功能与其他HtrA家族成员进行比较,并建模其催化过程.

主要方法:

高分辨率结构确定psMucD.
对psMucD寡合化的基于溶液的分析.
与同类HtrA蛋白酶的序列和结构比较.
对psMucD催化机制的建模.

主要成果:

结构显示psMucD是一种典型的HtrA血清蛋白酶,具有不同的蛋白酶和PDZ域.
psMucD存在于溶液中的三元体,其N端环介导的三元化.
序列和结构分析突出显示了与其他HtrA蛋白酶相比保存和独特的特征.

结论:

psMucD是一种结构特征的HtrA家族血清蛋白酶,来自Pseudomonas syringae.
它的三聚体结构由N端环介导,对其功能至关重要.
了解psMucD提供了对HtrA蛋白酶机制及其在细菌病原性中的作用的洞察.

关键词:

抗菌剂抗菌剂是一种抗菌剂.晶体结构晶体结构 MucD MucD MucD 是一个词.周周等离子体血清蛋白酶.伪蒙的注射器

更多相关视频

Transmembrane Domain Oligomerization Propensity determined by ToxR Assay

Transmembrane Domain Oligomerization Propensity determined by ToxR Assay

Published on: May 26, 2011

Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis

Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis

Published on: October 28, 2016

相关概念视频

Mechanism of Conjugation

Mechanism of Conjugation

Bacterial conjugation is a mechanism of horizontal gene transfer that enables the exchange of genetic material between bacterial cells through direct contact. This process is facilitated by a donor cell carrying a conjugative plasmid, which encodes genes necessary for pilus formation, DNA replication, and transfer. The conjugative plasmid plays a central role in initiating and executing the transfer of genetic material.The tra region of the conjugative plasmid encodes proteins responsible for...

Archaeal Cell Wall

Archaeal Cell Wall

Archaeal cell walls are structurally and compositionally distinct from their bacterial counterparts, lacking the characteristic peptidoglycan layer found in most bacteria. Instead, archaeal cell walls exhibit remarkable diversity, utilizing materials such as pseudomurein, polysaccharides, and proteins to construct their protective outer layers. This structural flexibility is closely tied to archaea's ecological adaptability.S-Layers: The Common Archaeal Cell WallThe S-layer is the most...

Insertion of Single-pass Transmembrane Proteins in the RER

Insertion of Single-pass Transmembrane Proteins in the RER

Integral membrane proteins are proteins adhered to the lipid bilayer of a cell organelle or membrane. They can be of two types: transmembrane integral proteins that span the lipid bilayer and monotopic proteins that are attached to either side of the membrane but do not pass through it.
Integral transmembrane proteins possess transmembrane and extra membrane domains. The transmembrane domains are primarily made of 20-25 hydrophobic amino acids arranged in a helical secondary confirmation. These...

Insertion of Multi-pass Transmembrane Proteins in the RER

Insertion of Multi-pass Transmembrane Proteins in the RER

The rough ER membrane synthesizes, assembles, and embeds transmembrane proteins in diverse topologies. These proteins function as transporters or channels and can remain in the ER membrane or are sent to the Golgi complex, lysosome, and cell membrane.
The multipass transmembrane proteins are the type IV integral membrane proteins with multiple topogenic sequences determining their spatial arrangement in the ER membrane. Nearly all multipass proteins lack a cleavable signal sequence and use...

Structure of Porins

Structure of Porins

Mitochondria, chloroplasts, and gram-negative bacteria have transmembrane, beta-barrel proteins called porins to mediate the free diffusion of ions and metabolites across the membrane. Mitochondrial porin precursors contain conserved amino acid sequences called beta signals at their C-terminal. Beta signals have a motif of PoXGXXHyXHy (Po-Polar, X-Any amino acid, G-Glycine, Hy-LargeHydrophobic), which are crucial for precursor recognition to initiate precursor assembly. Beta-barrel...

Export of Misfolded Proteins out of the ER

Export of Misfolded Proteins out of the ER

After folding, the ER assesses the quality of secretory and membrane proteins. The correctly folded proteins are cleared by the calnexin cycle for transport to their final destination, while misfolded proteins are held back in the ER lumen. The ER chaperones attempt to unfold and refold the misfolded proteins but sometimes fail to achieve the correct native conformation. Such terminally misfolded proteins are then exported to the cytosol by ER-associated degradation or ERAD pathway for...