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Dsup协调谷物发育和中的非生物压力

Chanjuan Ye¹, Jie Guo¹, Xin-Qiao Zhou¹

¹Rice Research Institute, Guangdong Rice Engineering Laboratory, Guangdong Academy of Agricultural Sciences, Key Laboratory of Genetic and Breeding of High Quality Rice in Southern China (Co-construction by Ministry and Province), Ministry of Agricultural and Rural Affairs, Guangzhou, 510640, China.

Plant physiology and biochemistry : PPB

|November 17, 2023

概括

迟的DNA损伤抑制蛋白Dsup增强了作物对辐射的抵抗力,并改善了农业特征. Dsup-overexpressing米表现出增加的应力耐受性和改变的谷物特征,表明其在作物育种方面的潜力.

科学领域:

植物生物学植物生物学
遗传学是一个遗传学.
生物技术是生物技术.

背景情况:

DNA损伤对有机体构成重大威胁,环境压力因素加剧了这种威胁.
迟缓的DNA损伤抑制蛋白 (Dsup) 在人类细胞和烟草中表现出保护作用.
在提高农作物抗辐射能力方面,DSup的有效性在很大程度上仍未被探索.

研究的目的:

研究Dsup过度表达对DNA损伤抵抗和作物的农学特征的影响,特别是大米.
阐明Dsup在植物中的功能背后的分子机制.
评估Dsup在提高作物对辐射和非生物应激应力的抵抗力方面的潜力.

主要方法:

在大米中使用Agrobacterium介导的转化生成稳定的Dsup过度表达线.
通过扫描电子显微镜进行农学特征分析,包括种子和幼苗阶段,颗粒大小和粉颗粒结构.
RNA测序 (RNA-seq) 用于分析与辐射和非生物压力相关的基因表达变化.
用液体染色学-并联质谱法 (IP-LC-MS) 进行免疫沉丰富,以识别Dsup相互作用的蛋白质.

主要成果:

Dsup过度表达增强了大米种子和幼苗的DNA损伤抵抗力.
过度表达Dsup的水植物显示出增大颗粒大小和改变粉颗粒结构和细胞大小.
RNA-seq分析表明,Dsup植物中与辐射相关的和与非生物压力相关的基因的表达增加.
IP-LC-MS确定了21种与DSup相互作用的蛋白质,其中许多参与转录和翻译.

结论:

Dsup增强了DNA损伤的抵抗力,并改善了大米的关键农学特征.
Dsup似乎通过与转录和翻译机械相互作用来调节DNA保护和植物发育之间的平衡.
Dsup显示了开发具有增强抗辐射和无生物应激耐受性的作物的潜力,为作物育种提供了新的见解.

关键词:

造成的DNA损伤是DNA损伤.破坏抑制蛋白质的破坏抑制蛋白质.粒度大小粒度大小粒度大小拉马佐蒂斯 (Ramazzottius varieornatus) 是一个多彩的鸟类.转基因大米是一种转基因大米.紫外线的压力压力是紫外线的压力.

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