灵活链改进的DNAMOF作为一种多功能平台,用于在葡萄糖催化过程中的化学酶级联反应
Yuan Rao1, Wei Zhuang1, Jinle Liu2
1College of Biotechnology and Pharmaceutical Engineering, State Key Laboratory of Materials-Oriented Chemical Engineering, National Engineering Technique Research Center for Biotechnology, Nanjing Tech University, No. 30, Puzhu South Road, Nanjing 211816, China; School of Chemical Engineering, Zhengzhou University, Zhengzhou 450001, China.
Enzyme and microbial technology
|November 17, 2023
概括
这项研究在MOF纳米片上固定葡萄糖氧化酶 (GOD),使用DNA定向固定. 这种方法提高了GOD的稳定性和活性,减少了生物催化剂应用的有毒副产品.
科学领域:
- 生物催化和生物技术
- 酶固定化 酶固定化
- 纳米材料是一种纳米材料.
背景情况:
- 葡萄糖氧化酶 (GOD) 对于葡萄糖的转化至关重要,产生葡萄糖酸和过氧化 (H2O2).
- 过氧化 (H2O2) 对GOD有毒,因此需要在酶级联反应中减轻其影响的策略.
- 酶级联反应通常涉及额外的成本和对酶活性,稳定性和可回收性进行复杂的优化.
研究的目的:
- 开发一种温和而多功能的策略,通过使用DNA导向固定 (DDI) 在氧基激活MOF (Cu-TCPP(Fe)) 上固定GOD.
- 利用MOF纳米片作为GOD固定的载体和H2O2分解的过氧化酶类催化剂.
- 提高固定GOD的稳定性,活动性和可回收性.
主要方法:
- 采用DNA定向固定 (DDI) 技术将GOD在氧基激活MOF (Cu-TCPP(Fe)) 纳米片上.
- MOF纳米片的作用是双重的:固定GOD和催化H2O2分解.
- 在各种条件下评估了酶活性,稳定性和可回收性.
主要成果:
- 固定的GOD在7个使用周期后保留了55.78%的初始活动.
- 在50°C保存96小时后,超过60%的固定酶的催化活性仍然存在.
- 基于MOF的固定化策略有效地减轻了H2O2.2的有害影响.
结论:
- 这项研究提出了一种新的方法,用于制备具有改善稳定性,快速扩散和高活性的固定酶.
- 开发的方法为生物催化剂和生物技术中的应用提供了一个有希望的策略.
- 双功能的MOF纳米片为酶固定和副产品管理提供了一个有效的平台.
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