多种Listeria monocytogenes内部克隆存在于一个动态的冷蔬菜加工环境中
Nadja Pracser1, Andreas Zaiser2, Hui Min Katharina Ying2
1FFoQSI GmbH-Austrian Competence Centre for Feed and Food Quality, Safety and Innovation, Technopark 1D, 3430 Tulln, Austria.
International journal of food microbiology
|November 17, 2023
概括
李斯特菌 (Listeria monocytogenes) 是一种食品安全问题,通常在冷蔬菜中发现. 这项研究确定了加工设施中持久的"内部"克隆,强调输送带作为污染源和WGS作为关键检测工具.
科学领域:
- 食品微生物学 食品微生物学
- 食品安全 食品安全
- 基因组学就是基因组学.
背景情况:
- 李斯特菌单细胞菌 (L. monocytogenes) 是一种与李斯特菌病爆发有关的重要食物传播病原体.
- 冷蔬菜已与最近的L. monocytogenes疫情有关,强调需要研究其在加工环境中的存在.
- 有限的研究存在于Listeria的发生和行为在冷蔬菜加工设施.
研究的目的:
- 调查L. monocytogenes和其他Listeria物种在冷蔬菜加工环境和产品中的流行情况.
- 用全基因组测序 (WGS) 识别和表征特定的L. monocytogenes菌株,包括"内部"克隆,使用全基因组测序.
- 评估与L. monocytogenes在这种环境中的生存和持久性相关的基因组和表型特征.
主要方法:
- 全基因组测序 (WGS) 用于多位置序列类型 (MLST) 和核心基因组MLST (cgMLST) 分析.
- 基因组比较分析和孤立物体的表型表征.
- 处理区的环境采样和产品测试.
主要成果:
- 菌种在加工环境中普遍存在,已经确定了五种不同的L. monocytogenes"内部"克隆.
- 输送带被确定为污染的主要来源.
- 隔离物具有抗压力标志物;ArgB存在与"内部"克隆有关,在低温下生长速度有所不同.
结论:
- WGS有效地追踪污染源并识别食品加工中的L. monocytogenes持续菌株.
- 该研究确定了冷蔬菜设施内的特定"内部"克隆和潜在的污染途径.
- 需要对微生物群和生物膜进行进一步的研究,才能充分理解L. monocytogenes的持久机制.
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