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一种GH81型β-葡萄糖结合蛋白增强了在大麦中的互利性真菌的殖民

Alan Wanke¹, Sarah van Boerdonk¹, Lisa Katharina Mahdi¹

¹Institute for Plant Sciences, University of Cologne, Cologne, Germany; Max Planck Institute for Plant Breeding Research, Cologne, Germany.

Current biology : CB

|November 17, 2023

概括

植物葡萄糖结合蛋白 (GBPs) 与真菌β-葡萄糖相互作用,调解植物免疫力. 发生突变的大麦GBPs减少了有益和致病真菌的殖民,增强了植物的防御能力.

科学领域:

植物与微生物的相互作用
植物免疫力植物免疫力
菌类细胞壁生物学菌类细胞壁生物学

背景情况:

植物细胞壁是对抗真菌病原体和共生体的关键障碍.
细胞壁的成分,如β-葡萄糖,可以触发植物的免疫反应.
糖糖结合蛋白 (GBPs) 参与识别真菌结构.

研究的目的:

为了识别与真菌β-葡萄糖相互作用的植物蛋白质.
研究一种特定的β-葡萄糖相互作用因子在植物和真菌相互作用中的作用.
了解葡萄糖结合蛋白1 (GBP1) 在大麦免疫中的作用.

主要方法:

使用生物化拉美林 (一种β-葡萄糖) 的蛋白质拉下测试.
通过质谱学识别相互作用蛋白质.
在大麦中对1英和2英的基因突变分析 (CRISPR/Cas9).
在大麦根和叶子中的真菌殖民和生物质的量化.

主要成果:

一种大麦糖化物化酶家族的81型葡萄糖结合蛋白 (GBP) 被确定为β-葡萄糖相互作用体.
大麦GBP1和GBP2的突变导致了有益的内植物 (Serendipita spp., Rhizophagus irregularis) 的殖民减少.
GBP突变还减少了病原体Bipolaris sorokiniana的生物量,并抑制了Blumeria hordei的透,增加了质沉积.

结论:

英1在调解植物对有益和致病菌的反应中起着至关重要的作用.
大麦GBPs参与识别真菌β-葡萄糖和调节宿主免疫力.
病原体可能已经演变为利用英中介识别途径成功殖民.

关键词:

大麦大麦大麦大麦大麦大麦大麦大麦细胞壁的细胞壁.兼容性兼容性兼容性的兼容性葡萄糖酶的使用方法葡萄糖是一种葡萄糖.东道主殖民化殖民化植物与真菌的相互作用.电阻的电阻是指电阻的电阻易感性易感性易感性这种共生是共生.

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