使用EDTA复合的超临界流体色谱-质谱分析金属离子的新方法
Kateřina Plachká1, Felix Bredendiek2, Lucie Nováková1
1Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Heyrovského 1203, 500 05, Hradec Králové, Czech Republic.
Analytica chimica acta
|November 17, 2023
概括
这项研究引入了新的超临界流体色谱-并联质谱法 (SFC-MS/MS) 方法,用于高效的金属离子分析. 这些绿色,快速的技术提供了可靠的重金属检测在各种矩阵,克服现有方法的局限性.
科学领域:
- 分析化学 分析化学
- 染色体学 染色体学 是一种染色学.
- 质谱测量质量谱测量
背景情况:
- 精确检测金属离子,特别是重金属,在环境,制药和毒理学应用中至关重要.
- 现有的分析方法经常面临光谱和化学干扰,需要开发更强大的技术.
- 超临界流体染色学与双重质谱学 (SFC-MS/MS) 相结合被探索为一种潜在的解决方案.
研究的目的:
- 研究SFC-MS/MS的适用性,用于使用基于CO2的移动相测定金属离子.
- 开发和比较使用不同带进行金属复合和分析的新方法.
- 建立一种快速,可靠和绿色的金属离子量化分析方法.
主要方法:
- 开发了三种不同的超高性能SFC-MS/MS (UHPSFC-MS/MS) 方法,采用配体:乙烯基二胺三酸 (EDTA),二甲基二甲酸 (DDC) 和乙乙酸 (acac).
- 使用高分辨率质谱仪 (HRMS) 合成和确认金属复合物 (Cu,Co,Cr,Fe,Al,Mn,Zn,Ni,Bi,Pb).
- 通过确定校准范围和注射可重复性来评估方法性能.
主要成果:
- 成功合成和HRMS确认了各种金属复合物与三个配体.
- 使用UHPSFC-MS/MS和短周期时间 (2-5分钟) 证明了金属复合物的高效分离.
- 验证了基于EDTA的方法对分析Ni,Bi和Pb等额外金属离子的普遍性.
结论:
- 这项研究报告了UHPSFC-MS/MS的首次应用,用于用EDTA分析金属复合物.
- 开发的UHPSFC-MS/MS方法为金属离子分析提供了快速,高效和环保的替代方案.
- 基于SFC的分离显示了扩展的适用性,提供了一种有希望的方法来克服当前分析技术中的干扰.
关键词:
乙氨基酸是什么 乙氨基酸甲基二甲基二甲基碳酸盐二甲基二甲基二甲基二甲基碳酸盐二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基二甲基艾迪塔 (EDTA) 是一个叫做EDTA的语言.质谱测量质量谱测量金属离子复合体是金属离子复合体.超临界流体色谱学 超临界流体色谱学更多相关视频
相关概念视频
01:30EDTA: Direct, Back-, and Displacement Titration
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The EDTA titration types for metal ion analysis include direct titration, back-titration, and replacement titration.
Direct titration involves buffering the metal ion solution to the desired pH and directly titrating with standard EDTA until the endpoint. The optimum pH ensures a large conditional formation constant of metal−EDTA and visibility of the free indicator color in the solution. In addition, auxiliary complexing reagents are used to prevent the precipitation of metal hydroxides...
Direct titration involves buffering the metal ion solution to the desired pH and directly titrating with standard EDTA until the endpoint. The optimum pH ensures a large conditional formation constant of metal−EDTA and visibility of the free indicator color in the solution. In addition, auxiliary complexing reagents are used to prevent the precipitation of metal hydroxides...
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01:18Effects of EDTA on End-Point Detection Methods
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Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
277
01:26EDTA: Auxiliary Complexing Reagents
596
EDTA titrations are usually carried out in highly basic conditions, where the fully deprotonated form of EDTA, Y4−, actively complexes with the free metal ions in the solution. Several metal ions precipitate as hydrous oxide (hydroxides, oxides, or oxyhydroxides) under these conditions, lowering the concentration of free metal ions in the solution. For this reason, auxiliary complexing agents or ligands such as ammonia, tartrate, citrate, or triethanolamine are used in EDTA titrations to...
596
00:56Extraction: Advanced Methods
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Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
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01:30Capillary Electrophoresis: Applications
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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
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01:23EDTA: Indirect and Alkalimetric Titration
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Unlike direct titration, back-titration, and displacement titration, indirect titration is an EDTA titration method for quantifying anions. In the indirect titration method, anions are precipitated as their insoluble salts with excess metal ions. The filtrate containing the excess metal ions is directly titrated with standard EDTA until the endpoint is achieved. Another approach involves extracting the metal ion and back-titrating with standard EDTA to obtain the endpoint. In this way, the...
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