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开发基于烯的全细胞生物传感器,用于可视检测微量爆炸物和重金属

Meijie Li¹, Shuzhe Lv¹, Rumeng Yang¹

¹Energy-rich Compound Production By Photosynthetic Carbon Fixation Research Center, Shandong Key Lab of Applied Mycology, College of Life Sciences, Qingdao Agricultural University, No. 700 Changchen Road, Qingdao, 266109, PR China.

Analytica chimica acta

|November 17, 2023

概括

在大肠杆菌 (Escherichia coli) 中,一种新的基于烯的全细胞生物传感器为检测2,4-丁二 (DNT),一种危险的爆炸性残留物提供可见信号. 这项创新使埋藏爆炸物的视觉检测成为可能,提高了冲突地区的安全.

科学领域:

生物技术是生物技术.
环境科学环境科学
化学工程是化学工程的重要组成部分.

背景情况:

剩余爆炸物对人类安全和环境构成重大风险.
现有的全细胞生物传感器用于2,4-丁二 (DNT) 等爆炸物,依赖于光或发光,阻碍了现场检测.
对于现实世界的应用,需要具有易于检测的输出信号的生物传感器.

研究的目的:

开发一种全细胞生物传感器,用于2,4-丁二 (DNT) 产生可见信号.
设计一种生物传感器,用于视觉检测埋藏的爆炸物.
为了提高生物传感器的灵敏度和稳定性,以便在实践中使用.

主要方法:

使用大肠杆菌 (Escherichia coli) 构建了一个全细胞生物传感器,其中包含了DNT反应的促进物yqjF和烯合成基因盒crtEBI.
优化了新陈代谢流量,以增强利科的生产,并使用终止器来最大限度地减少背景噪音.
已验证的生物传感器性能,包括DNT特异性,环境稳定性和土壤样本中的检测.

主要成果:

优化的生物传感器LSZ05显示能够检测至少1mg/L的DNT.
证实了DNT的特异性和在各种环境条件下强大的性能.
在两种类型的土壤样本中成功检测到DNT,并显示了重金属检测的潜力.

结论:

一个基于柳科的全细胞生物传感器 (LSZ05) 已成功开发,用于对DNT的视觉检测.
软化证明是储存生物传感器的有效方法.
这种可见信号生物传感器为在具有挑战性的环境中检测埋藏的爆炸物和潜在的重金属提供了宝贵的进步.

关键词:

2,4-丁尼特二烯 (DNT) 是一种爆炸物是一种爆炸物.这种药物是百合素 (Lycopene).视觉检测视觉检测是一种视觉检测.全细胞生物传感器

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