由可转移元素启动的增强剂类元素产生了多倍体小麦的亚基因组偏差尖峰特异性
Yilin Xie1,2,3, Songbei Ying1, Zijuan Li2,3
1State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, 200438, China.
Nature communications
|November 17, 2023
概括
在小麦基因组中,可转移元素 (TE) 产生增强剂类元素 (ELE),产生调节性RNA (ELE-RNA). 这些ELE-RNAs,特别是来自RLG_famc7.3的ELE-RNAs,控制尖端发育和多倍体可塑性.
科学领域:
- 基因组学就是基因组学.
- 植物生物学 植物生物学
- 进化生物学 进化生物学
背景情况:
- 可转移元素 (TE) 构成了普通小麦基因组的很大一部分.
- 跨子基因组的TE多样性表明在多倍体可塑性中发挥了作用,尽管因果关系仍然未被证明.
研究的目的:
- 为了研究TE在调节常见小麦的基因表达和多倍体可塑性中的功能作用.
- 识别和表征来自TE及其相关转录的增强剂类元素 (ELEs).
主要方法:
- 整合基因表达数据 (上限分析) 和表观基因组概况 (隐藏的马尔科夫模型).
- 在不同发育阶段对增强剂类元素衍生RNA (ELE-RNA) 转录组的量化.
- 对转录因子结合和基因淘汰实验的分析.
主要成果:
- 有相当数量的TE作为增强剂类元素 (ELE) 起作用,产生非编码转录 (ELE-RNA).
- 主要来自亚基因组A中的RLG_famc7.3的TE启动的ELE-RNA与尖端特异性基因表达有关.
- 这些ELE-RNAs的淘汰导致下调的尖端特异性基因和异常的尖端发展.
结论:
- 试验特质有助于小麦的监管特异性和多倍体发育可塑性.
- 由TE驱动的监管创新在多体进化中起着至关重要的功能作用.
- 特定的TE家族,如RLG_famc7.3,可以用于作物发育中的向基因调节.
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