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相关概念视频

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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相关实验视频

Updated: Jul 10, 2025

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells
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单镜头动态扫描,用于同时在现场检测位置和激光处理焦点控制.

Xiaohan Du1,2, Camilo Florian3,4, Craig B Arnold1,4

  • 1Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ 08544 USA.

Light, science & applications
|November 18, 2023
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概括

这项研究引入了一种新的单镜头自动聚焦方法,用于激光处理. 它可以同时实现高速表面检测和Z轴调整,克服先前先进制造中的速度限制.

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科学领域:

  • 光学和光子学 在光学和光子学.
  • 先进制造先进制造 制造先进制造
  • 精密工程 精密工程是指精密的工程.

背景情况:

  • 传统的激光处理自动聚焦依赖于单独的,缓慢的表面检测和Z轴调整模块.
  • 机械Z轴阶段限制了处理速度,在高吞吐量制造中造成了瓶.
  • 现有的方法很难跟上快速的横向处理速度.

研究的目的:

  • 开发一个更快,集成的自动对焦系统,用于激光处理.
  • 为了实现近同步的表面检测和Z轴调整.
  • 为了加速需要精确3D定位的先进制造工艺.

主要方法:

  • 一种新的单镜头方法,在双光束设置中使用高速Z扫描光学元件.
  • 通过扫描表面的探测束和对焦光学检测进行现场表面检测.
  • 动态表面检测在140-350 kHz实现,焦点控制与扫描镜头振荡同步.

主要成果:

  • 证明了具有可控范围,高可重复性和1.10%的线性误差的动态表面检测.
  • 在140-350 kHz实现了近似同时的表面跟踪和对焦控制.
  • 与传统方法相比,大大减少了轴向对齐时间.

结论:

  • 开发的单镜头方法克服了激光处理自动聚焦的速度瓶.
  • 这种方法可以实现瞬间的表面跟踪和精确的焦点对齐.
  • 这种方法具有显著的潜力,可以提高3D先进制造的速度和效率.