从咖啡酸中微生物生产植物黄 hesperetin
Erik K R Hanko1, João Correia2, Caio S Souza2
1Manchester Institute of Biotechnology, Faculty of Science and Engineering, University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK.
BMC research notes
|November 18, 2023
概括
在大肠杆菌 (E. coli) 中从咖啡酸中实现了hesperetin的微生物生产. 酶工程提高了hesperetin的特异性,为这种制药类黄类药物提供了植物提取的潜在替代方案.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 酶工程是什么? 酶工程是什么?
背景情况:
- 类植物中的黄类化合物hesperetin具有制药潜力.
- 植物提取产生复杂的混合物,使隔离复杂化.
- 微生物生物合成提供了一个更纯粹的替代方案.
研究的目的:
- 在大肠杆菌 (E. coli) 中产生素.
- 结合埃里奥迪克提醇通路与黄类O-甲基转移酶.
- 提高CCoAOMT7的位置特异性,用于产生素.
主要方法:
- 在大肠杆菌中利用了先前优化的埃里奥迪克提醇生物合成途径.
- 选了一个组合式库的黄类O-甲基转移酶.
- 采用酶工程与同质模型和对接模拟.
主要成果:
- 在CCoAOMT7中,产生了14.6 mg/L的hesperetin和3.8 mg/L的homoeriodictyol. 在CCoAOMT7中,产生了14.6 mg/L的hesperetin和3.8 mg/L的homoeriodictyol.
- 确定了一种CCoAOMT7突变,其hesperetin特异性高出两倍.
- 突变酶显示催化活性降低.
结论:
- 赫斯佩雷丁可以从大肠杆菌的中央碳代谢中合成.
- 酶工程可以提高黄类O-甲基转移酶的特异性.
- 微生物生产是制药类黄类药物的可行途径.
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