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Updated: Aug 5, 2026

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内部ELISA协议用于体p24检测各种艾滋病毒分离物

Mariano A Molina1, Monique Vink1, Ben Berkhout1

  • 1Department of Medical Microbiology, Laboratory of Experimental Virology, Amsterdam UMC, AMC Location, University of Amsterdam, Amsterdam, The Netherlands.

Virology journal
|November 18, 2023
PubMed
概括

研究人员开发了具有成本效益的内部酶相关免疫吸收试验 (ELISA) 来检测人类免疫缺陷病毒 (HIV) 体p24 (CA-p24) 抗原. 这些测试为HIV-1研究提供了有价值的替代方案,提供高特异性和灵敏度.

关键词:
抗体是一种抗体.抗原是一种抗原.在 CA-p24 中使用.艾丽莎·伊丽莎·伊丽莎艾滋病病毒 艾滋病病毒 艾滋病病毒

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.