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调节 miR-339-3p/COL1A1 的 hsa_circ_0020378 促进骨髓瘤的进展

Liang Liu^1,2, Wenze Huang^1,2

¹Department of Spinal Surgery, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, Huangshi, Hubei, China.

Cancer biology & therapy

|November 18, 2023

概括

循环RNA hsa_circ_0020378 (circ_0020378) 通过海绵化miR-339-3p和上调调节COL1A1.1,促进骨肉瘤. 沉默circ_0020378抑制了瘤的进展,为骨髓瘤提供了潜在的治疗点.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

骨髓瘤是一种流行的儿科骨癌.
循环RNAs (circRNAs) 在骨髓瘤的发展中起作用.
在骨髓瘤中hsa_circ_0020378 (circ_0020378) 的特定功能尚不清楚.

研究的目的:

研究circ_0020378在骨髓瘤进展中的作用和机制.
为了探索circ_0020378,miR-339-3p和COL1A1在骨髓瘤中的关系.

主要方法:

定量实时PCR和西式斑点测量基因和蛋白质表达.
细胞增殖,迁移和殖民地形成的测试.
在体内异种移植实验和分子相互作用研究 (露西法酶,RIP).

主要成果:

在骨髓瘤组织和细胞中,Circ_0020378和COL1A1的上调,而miR-339-3p的下调.
Circ_0020378 沉默抑制了骨髓瘤细胞的增殖,迁移和殖民地形成 in vitro 和 in vivo.
Circ_0020378 作为 miR-339-3p 的海绵,该海绵的目标是 COL1A1 3'UTR.

结论:

Circ_0020378通过抑制miR-339-3p/COL1A1轴来促进骨髓瘤的进展.
Circ_0020378是骨髓瘤治疗的潜在治疗点.

关键词:

在 COL1A1A1 中,环_0020378 环_0020378 环_0020378 环_0020378 环_0020378 环_0020378 环_0020378 环_0020378 环_0020378 环在 miR-339-3p 中使用.骨质肉瘤是骨质肉瘤的一种疾病.

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