酒消耗谷物的有效降解通过一种新型的恒温GH10氨酶
Mengyu Liu1, Shuxia Huang2, Peng Yan2
1State Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University, 72 Binhai Road, Jimo District, Qingdao, Shandong, 266237, China.
Applied biochemistry and biotechnology
|November 18, 2023
概括
酒消耗谷物 (BSG) 可以使用新型GH10氧化酶,SsXyn10A.有效降解. 这种热稳定酶是由Streptomyces sp.分泌的. F-3,显示了生物质水解和BSG价值化的潜力.
科学领域:
- 生物技术是生物技术.
- 酶学 是一种酶学.
- 工业微生物学 工业微生物学
背景情况:
- 酒消耗谷物 (BSG) 是丰富的,易腐烂的副产品,需要有效降解以获得环境和经济效益.
- 杆菌种 (Streptomyces) sp. 这种杆菌种 (Streptomyces) 是一种 F-3使用BSG作为唯一的碳和源,表明酶生产的潜力.
研究的目的:
- 为了识别和描述来自Streptomyces sp.的酶. F-3 能够降解 BSG 的性能.
- 评估用于生物质水解的新型GH10氧化酶 (SsXyn10A) 的性能和应用潜力.
主要方法:
- 蛋白质组质谱法被用来识别来自Streptomyces sp.的分泌蛋白质. 在BSG上种植的F-3.
- 确定GH10西兰酶 (SsXyn10A) 的生物化学特征,包括最佳活性,热稳定性和基质特异性.
- 使用SsXyn10A,单独和与细胞酶协同进行BSG的酶化水解测定,有或没有蒸汽爆炸预处理.
主要成果:
- 蛋白质组分析发现了一种高度分泌的GH10西兰酶,SsXyn10A.
- SsXyn10A在pH 7.0和60°C时表现出最佳活性,具有出色的热稳定性.
- SsXyn10A有效地化各种西兰和寡糖,并与细胞酶协同降解BSG,产生显著的降解糖,特别是在蒸汽爆炸预处理后.
结论:
- 从Streptomyces sp.中发现了一种新型的,恒温稳定的GH10氧化酶 (SsXyn10A),并进行了鉴定和特征化. 在F-3.0中,F-3是F-3.
- SsXyn10A显示了酒厂使用的谷物和其他菌纤维素生物质的高效水解的巨大潜力.
- 这项研究有助于造副产品的价值化和生物质转化工业酶的开发.
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