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来自传统发酵大豆糊 (大江) 的新乌玛米的识别,表征和受体结合机制
Kaixin Cao1,2, Feiyu An1,2, Junrui Wu1,3
1College of Food Science, Shenyang Agricultural University, Shenyang, Liaoning 110866, People's Republic of China.
Journal of agricultural and food chemistry
|November 18, 2023
概括
研究人员在大江中发现了新的乌玛米,这是一个发酵的大豆调味料. 这些,像HSISDLQ一样,对其美味的乌玛米味道和风味特征作出了重大贡献.
科学领域:
- 食品科学 食品科学 食品科学
- 生物化学 生物化学
- 感官科学 感官科学
背景情况:
- 大江是一种传统的中国发酵大豆调味品,因其乌玛米风味而受到重视.
- 乌玛米被认为是大江这种令人渴望的味道的主要来源.
- 了解特定的和它们的相互作用对于味道科学至关重要.
研究的目的:
- 为了阐明大江的乌玛米味道背后的机制.
- 为了识别和描述对大江的风味负责的新型乌玛米.
- 为了研究这些与味觉受体的相互作用.
主要方法:
- 使用乙醇沉,凝染色学和染色学-质谱学净化和识别.
- 选,合成,和新型豆乌玛米的特征.
- 感觉值的确定和分子对接模拟.
主要成果:
- 发现并合成了三种新的乌玛米 (TLGGPTTL,GALEQILQ,HSISDLQ).
- 对于每个 (0.25,0.40,0.17 mmol/L) 确定了感觉值.
- 分子对接揭示了与T1R3味觉受体残留物Ser147和Glu148.8的结和疏水相互作用.
结论:
- 这项研究成功地确定了大江的关键乌玛米.
- 这些发现揭示了发酵大豆产品中由乌玛米驱动的风味的分子机制.
- 识别的和它们的相互作用提供了对味觉受体功能和食物风味发展的洞察.
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