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转化生长因子-β1调解SMAD4/BMF通路,以调节小尾汉羊的卵巢颗粒细胞亡

Mingna Li¹, Weiwei Liang¹, Yuzhu Luo¹

¹Gansu Key Laboratory of Herbivorous Animal Biotechnology, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, 730070, China.

|November 18, 2023

概括

转化生长因子-β1 (TGF-β1) 促进绵羊卵巢中的颗粒细胞增殖和活力. 它通过SMAD4/BMF通路抑制细胞亡,帮助卵泡发育.

科学领域:

生殖生理学生殖生理学
细胞生物学细胞生物学
分子内分泌学分子内分泌学

背景情况:

转化生长因子β1 (TGF-β1) 是TGF-β信号传递中的关键细胞因子,调节女性生殖功能,如卵泡发育.
在小尾汉羊的毛囊发育过程中,TGF-β1在颗粒细胞增殖和亡中的特定作用尚不清楚.

研究的目的:

为了研究羊卵巢在卵泡阶段TGF-β1的表达.
为了确定TGF-β1对绵羊颗粒细胞的活力,增殖和亡的影响.
阐明TGF-β1对粒状细胞的作用所涉及的分子途径.

主要方法:

在羊卵巢中分析TGF-β1mRNA和蛋白质表达.
在体外测试 (细胞计数套件-8,EDU标记,流动细胞计量) 以评估颗粒细胞的活力,增殖和亡.
针对SMAD4的RNA干扰,以调查途径的参与.

主要成果:

卵巢中的TGF-β1表达在雌性期和排卵前期之间有所不同.
外源TGF-β1 (5和10 ng/mL) 增强了颗粒细胞的活力和增殖,同时降低了细胞亡和Bcl-2-修饰因子 (BMF) 表达.
SMAD4干扰逆转了TGF-β1的抗亡作用,并增加了BMF表达.

结论:

TGF-β1通过SMAD4/BMF通路抑制细胞亡,促进小尾汉羊的颗粒细胞增殖和活力.
这种机制对卵巢卵泡活动至关重要,并表明TGF-β1是调节卵泡发育的潜在目标.

关键词:

毛囊 (Follicles) 是一个毛囊.颗粒素细胞是颗粒素的细胞.小尾的汉族羊小尾的汉族羊在TGF-β1中.

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