植入式生物材料的表面光杀菌:结构和功能表征
Sahand Ghaffari1, Nafise Amiri2, Demian F Felix1
1The Stone Centre at VGH, Department of Urologic Sciences, University of British Columbia, Jack Bell Research Centre, 550-3, 2660 Oak Street, Vancouver V6H 3Z6, BC, Canada.
Colloids and surfaces. B, Biointerfaces
|November 18, 2023
概括
使用聚甲基 (PDMS) 和嵌入式光敏感剂的新型光动力学灭菌方法有效地防止医疗植入物上的细菌殖民和生物膜形成,显著减少医院获得感染 (HAI). 这种具有成本效益的方法在体内表现出强大的抗菌活性,没有任何不良影响.
科学领域:
- 生物材料科学 生物材料科学
- 传染病控制和控制传染病
- 光动力学疗法 光动力学疗法
背景情况:
- 在医院获得的感染 (HAI),特别是导管相关的尿路感染 (CAUTIs),构成了全球医疗保健的重大挑战.
- 抗生素耐药性增加需要采用替代策略来对抗与内置医疗设备相关的反复感染.
- 植入物表面上的细菌粘附和生物膜形成是CAUTIs和相关患者发病率的主要驱动因素.
研究的目的:
- 开发一种简单,具有成本效益的方法来消毒基植入物表面.
- 使用光动力学作用防止在聚甲基 (PDMS) 上的细菌殖民和生物膜形成.
- 评估一种新的PDMS嵌入式光敏化剂 (PS) 系统的疗效和安全性,用于预防感染.
主要方法:
- PDMS表面嵌入了一种光敏化剂 (PS).
- 该PDMS+PS材料暴露在LED光线下以激活光杀.
- 评估了细菌粘附,生物膜形成,体内疗效,细胞毒性和材料特性.
主要成果:
- 光动力治疗导致24小时内杀菌活性低于检测极限 (<10 CFU/mL).
- 活/死染色证实了表面细菌和生物膜形成的显著减少.
- 在体内研究表明,与3天的对照相比,植入PDMS+PS优惠券的附着细菌减少了99.5%.
- 组织学和体外细胞毒性研究没有发现任何不良反应或细胞毒性.
- 表面处理的PDMS表现出与裸体PDMS相似的或改进的材料特性 (拉伸强度,粗性,疏水性,层).
结论:
- PDMS+PS系统为医疗植入物提供了一种强大,具有成本效益的光动力学灭菌策略.
- 这种方法有效地防止了细菌殖民和生物膜的形成,这对于减少CAUTI和HAI至关重要.
- 该材料具有生物相容性,并保持或增强可取的物理性能,使其适合临床应用.
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