玉米ZmBES1/BZR1-1转录因子对干旱耐受性的负面调节
Wenqi Feng1, Hongwanjun Zhang1, Yang Cao1
1Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Maize Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.
Plant physiology and biochemistry : PPB
|November 18, 2023
概括
玉米ZmBES1/BZR1-1基因增强了植物对干旱的敏感性. 过度表达会降低生存率和水分含量,这表明干旱应激反应中的负面调节作用.
科学领域:
- 植物分子生物学 植物分子生物学
- 无生物应激反应应激反应
背景情况:
- 干旱压力显著限制了作物生产率,需要对与压力相关的基因进行研究.
- BRI1-EMS1抑制剂 (BES1) /耐巴西纳1 (BZR1) 转录因子是植物生长和压力的关键调节者.
- 玉米BES1/BZR1基因在压力耐受性方面的作用尚未得到充分探索.
研究的目的:
- 在干旱应激反应中克隆和功能性描述玉米ZmBES1/BZR1-1基因.
- 研究ZmBES1/BZR1-1在干旱耐受性中的作用背后的分子机制.
主要方法:
- 基因克隆和功能性表征在转基因阿拉比多普西斯和大米中.
- 分析蛋白质定位,转录活性和基因表达模式.
- 在干旱压力下进行生理分析 (生存率,ROS,RWC,口腔孔径,REL).
- RNA测序 (RNA-seq) 和基因本体学 (GO) 分析以确定目标基因和途径.
主要成果:
- ZmBES1/BZR1-1蛋白具有保存的bHLH域,定位到核中,并表现出转录激活.
- 在阿拉比多普西斯和大米中过度表达ZmBES1/BZR1-1增加了干旱敏感性,以减少生存率,较低的ROS和RWC以及增加的口腔孔径和REL.
- 通过RNA-seq确定了56个由ZmBES1/BZR1-1调节的差异表达基因,主要涉及对氧化应激和氧气水平的反应.
结论:
- ZmBES1/BZR1-1作为玉米干旱应激反应的负调节剂.
- 这项研究提供了关于BZR1/BES1介导干旱应激反应的分子机制的见解.
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