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通过在发酵过程中使用 Priestia megaterium 的非典型代谢转移将甘生物转化为多基酸盐:对碳和源度的统计分析

Salma Shahid¹, Ridha Mosrati², David Corroler²

¹Department of Biochemistry, Government College Women University, Faisalabad, Pakistan.

International journal of biological macromolecules

|November 18, 2023

概括

普里斯蒂亚巨 (Priestia megaterium) 生产中长链多酸 (PHA) 和新型混合物. 优化的条件产生了高的PHA产量,证明了先进生物塑料应用的潜力.

科学领域:

生物技术是生物技术.
微生物学微生物学
聚合物科学聚合物科学

背景情况:

聚酸酸盐 (PHA) 是由 prokaryotes 生产的可生物降解的生物塑料.
普里斯蒂亚巨被称为短链PHA (scl-PHA) 生产.
这项研究调查了P. megaterium. P. 产生的mcl-PHA和混合PHA的产生.

研究的目的:

描述P. 巨对于中长链PHA (mcl-PHA) 的生产.
开发具有增强性质的新型PHA混合物.
为工业应用优化微生物PHA生产.

主要方法:

统计分析和模拟以建模细胞干量和PHA含量.
优化微生物生长条件以获得PHA产量.
福里埃变换红外光谱 (FTIR) 用于PHA量化.
气色谱-火焰电离检测 (GC-FID) 用于PHA单体分析.

主要成果:

在优化条件下,最大PHA产量为0.05gg-1.H-1.
最大PHA生产率达到0.28g.L-1.H-1的度为4.94g.L-1.1.
FTIR被验证为用于PHA监测的半定量工具.
证明了scl-PHA随着时间的推移转化为mcl-PHA.

结论:

可以被设计为mcl-PHA和混合PHA生产.
优化的发酵条件显著提高了PHA产量和生产率.
这项研究支持开发具有定制性质的新型生物塑料.

关键词:

生物质是生物质中的一部分.生物聚合物的生物聚合物.生物反应器是一种生物反应器.糖是一种糖.聚酸酸多基甲酸普里斯蒂亚巨动物群 (Priestia megaterium) 是一个巨动物群.

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