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石墨烯氧化物影响了塑体介导的抗生素耐药性基因转移到植物中
Ran Wu1, Jin Fang2, Xiaobo Xiang1
1Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, Interdisciplinary Research Academy (IRA), Zhejiang Shuren University, Hangzhou 310015, China.
The Science of the total environment
|November 18, 2023
概括
氧化石墨烯 (GO) 显著减少了抗生素耐药性基因 (ARG) 从细菌转移到大米植物,通过损害细菌数量和诱导植物氧化应激. GO还阻碍了ARG从根部迁移到地面植物部位的迁移.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 植物科学 植物科学
背景情况:
- 抗生素耐药性基因 (ARG) 是新出现的污染物,构成公共卫生风险.
- 石墨烯氧化物 (GO) 越来越多地进入环境,并可能对生态产生影响.
- 对GO对ARG从细菌转移到植物的影响仍然不太清楚.
研究的目的:
- 调查GO对RP4等离子体携带的ARG从Bacillus subtilis转移到大米植物中的影响.
- 阐明GO在细菌植物系统中对ARG迁移的影响的机制.
主要方法:
- 使用Bacillus subtilis和RP4等离子体在暴露于不同GO度 (0-400毫克L-1) 的大米植物中研究了ARG转移.
- 通过显微镜评估了细菌活力,植物氧化应激标记 (埃文斯蓝色染色,MDA,NO,PAL活性),植物生长参数 (新鲜重量,素含量) 和GO细胞吸收.
- 通过共同种植实验在米中确定了潜在的RP4受体菌株.
主要成果:
- GO显著减少了13-71%的ARG转移到大米根,并削弱了RP4等离子体向地面部分的迁移.
- 较高的GO度降低了溶液的pH值,降低了抗生素耐药的细菌,并在大米中诱导氧化应激,对植物生长产生负面影响.
- 黄金原料进入了大米根细胞,但没有进入叶子中细胞. 细菌细菌,B. amyloliquefaciens和B. cereus被确定为潜在的RP4接受者.
结论:
- 石墨烯氧化物在细菌-大米植物系统中显著抑制ARG转移和迁移.
- 转基因的作用是通过减少细菌活力和诱导植物氧化应激来调节的,这突显了潜在的生态风险.
- 了解GO对ARG传播的影响对于评估环境和公共卫生问题至关重要.
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