鉴定和描述calreticulin作为一种新的等离子体受体受体的特征
Alamelu G Bharadwaj1, Gillian C Okura2, John W Woods3
1Departments of Pathology, Dalhousie University, Halifax, Nova Scotia, Canada; Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada.
The Journal of biological chemistry
|November 18, 2023
概括
卡尔雷丘林 (CRT) 作为一种新型的等离子体结合受体,调节等离子体的转化为等离子体. 这一发现为CRT在癌症进展中的作用提供了新的见解.
科学领域:
- 生物化学 生化学
- 细胞生物学 细胞生物学
- 分子医学是分子医学.
背景情况:
- 卡尔雷蒂库林 (CRT) 是内细胞网膜中的结合蛋白,在平衡和蛋白质折叠中具有已知的细胞内作用.
- 最近已经确定了CRT的细胞外功能,包括癌细胞入侵和巨介导的细胞分裂中的作用.
研究的目的:
- 为了发现卡尔雷蒂库林 (CRT) 的新型细胞外功能.
- 研究CRT作为等离子体结合受体的作用及其调节等离子体转化为等离子体的作用.
主要方法:
- 表面等离子体共振分析以确定与CRT的等离子体结合亲和力.
- 在CRT的存在下测量通过组织等离子体激活剂或尿素酶类型等离子体激活剂的等离子体激活.
- 在缺乏CRT的纤维细胞和缺乏CRT的癌细胞中分析等离子体生成.
- 在野生类型和CRT缺陷细胞中对线粒激素治疗的反应中评估等离子体生成.
主要成果:
- CRT显著刺激了等离子体的转化为等离子体.
- CRT以中等亲和度 (KD = 1.8μM) 结合等离子体,结合和激活被e-氨基酸抑制,这表明氨酸残留物参与.
- 降低氨酸残留的CRT变体显示了血原激活的降低.
- 缺乏CRT的细胞表现出显著减少的等离子体生成,在野生类型细胞中可以通过线粒激活.
结论:
- 卡尔雷蒂库林 (CRT) 作为一个等离子体结合受体,关键调节等离子体生成.
- CRT 增强等离子体蛋白质溶解活性的能力为其在癌症中既定的作用提供了潜在的机械联系.
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