小鼠的OA易受性部分由肠道微生物组介导,可通过微生物组移植转移,并与免疫表型变化有关
Emmaline Prinz1,2, Leoni Schlupp1, Gabby Dyson1
1Arthritis & Clinical Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA.
Annals of the rheumatic diseases
|November 18, 2023
概括
在MRL小鼠中的肠道微生物组可以预防骨关节炎 (OA). 转移这种微生物组可以降低骨关节炎的严重程度,并改变免疫细胞的特征,证明其具有保护作用.
科学领域:
- 微生物组研究的研究.
- 免疫学 免疫学 免疫学
- 骨关节炎 (OA) 研究
背景情况:
- 墨菲·罗斯大 (MRL) /MpJ小鼠菌株表现出对创伤后关节炎 (OA) 的固有保护.
- 微生物组在这种OA保护中的作用以前没有被研究过.
研究的目的:
- 为了描述MRL和野生类型 (B6) 小鼠之间的微生物群差异.
- 评估微生物移植对OA发展和进展的影响.
- 在OA保护的背景下,研究与微生物组相关的免疫类型.
主要方法:
- 从MRL移植到B6小鼠的Cecal微生物移植,在OA手术诱导之前和之后 (中介半月体的破坏 - - DMM).
- 评估OA组织病理学 (OARSI分数),肌炎和骨菌形成8周后的DMM.
- 16S rRNA基因测序用于微生物组分析和元基因组功能归因.
- 质量细胞测量用于免疫类型.
主要成果:
- 在DMM之前,从MRL移植到B6小鼠的微生物组移植显著降低了OA组织病理 (70%),突炎 (60%) 和骨菌得分 (30%).
- 在DMM (48小时) 后不久进行的移植也改善了OA的结果,但延迟移植 (1-2周) 没有.
- 移植小鼠的后代 (F1和F2代) 也显示出OA保护.
- 特定的细菌分类,如 * Lactobacillus * 和 * Rikenellaceae * ,与OA结果相关.
- 移植的小鼠表现出改变的免疫类型,包括减少双阴性T细胞和增加CD25+CD4+T细胞.
结论:
- 肠道微生物组在MRL小鼠中观察到的骨关节炎保护中发挥着重要作用.
- 微生物组移植是一种可行的方法来转移这种保护作用.
- 移植诱导了与OA保护相关的全身免疫类型变化.
相关概念视频
The Oral Microbiota
The oral microbiome includes a complex ecosystem comprising over 700 microbial species, identified through genomic sequencing and culture-based analyses to date. This community includes a core microbiome, found universally among individuals, and a variable component influenced by environmental factors such as diet, lifestyle, and host genetics. Site-specific conditions, including oxygen gradients, pH levels, and nutrient availability, determine the spatial distribution of these microorganisms...
Functions of the Gut Microbiota
The gut microbiota includes trillions of microorganisms that colonize the human gastrointestinal tract, including bacteria, archaea, viruses, and fungi. This complex ecosystem plays a critical role in maintaining intestinal and systemic health. Most of these microbes inhabit the large intestine, establishing a relatively stable and diverse community that contributes to gut homeostasis through various metabolic, immunological, and protective mechanisms.Dominant bacterial phyla, such as...
Dysbiosis of the Gut Microbiota
The human gut microbiome includes a diverse array of microbial species, including beneficial commensals and opportunistic pathogens, which interact to support host health. These microbes contribute to essential functions such as nutrient metabolism, immune system modulation, and maintenance of intestinal barrier integrity. However, disruptions to this equilibrium—referred to as dysbiosis—can have widespread physiological consequences.Dysbiosis is often characterized by reduced microbial...
Gut-Brain Axis
The gut–brain axis is a bidirectional communication system that connects the gastrointestinal tract and the brain. This interaction is mediated through multiple pathways, including the vagus nerve, hormonal signals, immune responses, and chemical messengers produced by gut microbes.Microbial Contributions to Brain FunctionGut microbiota contributes significantly to brain function by producing neuroactive compounds. These include neuroactive compounds that influence neurotransmitters such as...
Microbiota Modulation by Antibiotics
Antibiotics have revolutionized modern medicine by saving countless lives from bacterial infections. However, their widespread use has inadvertently harmed the delicate balance of the human gut microbiota. The gut microbiota, a complex community of bacteria, archaea, viruses, and fungi, plays a vital role in regulating metabolism, immune responses, and maintaining intestinal health. Antibiotics, especially broad-spectrum types, disrupt this ecosystem by eradicating both harmful and beneficial...


