在组织数据集上依赖MALDI-TIMS-MS生物成像
Steffen Heuckeroth1, Arne Behrens2, Carina Wolf1
1Institute of Inorganic and Analytical Chemistry, University of Münster, Münster, Germany.
Nature communications
|November 18, 2023
概括
空间离子移动计划详尽的碎片化 (SIMSEF) 通过添加关键的碎片化光谱来增强被困离子移动性光谱-质谱成像. 这使得在组织上可靠的化合物注释和空间代谢学中化学空间的映射成为可能.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 代谢学 代谢学 代谢学
背景情况:
- 捕获离子移动性谱学 (TIMS) 与质谱学 (MS) 成像相结合,提供了先进的空间代谢学.
- 在TIMS-MS成像中缺乏碎片化光谱 (MS2) 阻碍了对化合物的确定.
- 组织上的注释仍然是空间代谢学中的一个挑战.
研究的目的:
- 为TIMS-MS成像引入空间离子移动计划详尽碎片化 (SIMSEF).
- 用必要的MS2光谱来增强TIMS-MS成像数据集.
- 改进组织上的化合物注释和化学空间探索.
主要方法:
- 开发了SIMSEF,这是TIMS-MS成像的数据集依赖的获取策略.
- 系统分布的碎片化实验与多个碰撞能量.
- 将SIMSEF集成到开源软件MZmine中,用于数据处理和分析.
- 在大鼠大脑组织上利用矩阵辅助激光脱/电离 (MALDI) -TIMS-MS.
主要成果:
- SIMSEF成功地将TIMS-MS成像数据集与MS2光谱进行了增强.
- 在MZmine中通过光谱库匹配和基于规则的脂质注释启用了组织上的化合物注释.
- 通过分子网络,便于绘制已知和未知的化学空间.
- 演示了大鼠大脑组织部分的工作流程.
结论:
- SIMSEF显著提高了TIMS-MS成像的分析能力.
- 在空间代谢学中提供了可靠的组织上化合物注释的强大方法.
- 开源算法和数据处理管道为化学空间探索提供了宝贵的社区资源.
更多相关视频
09:38Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
Published on: November 26, 2013
14.2K
07:58Capturing Small Molecule Communication Between Tissues and Cells Using Imaging Mass Spectrometry
Published on: April 3, 2019
6.7K
相关概念视频
MALDI-TOF Mass Spectrometry
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Peptide Identification Using Tandem Mass Spectrometry
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Matrix-Assisted Laser Desorption Ionization (MALDI)
Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
Rapid Identification of Pathogens
MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...
