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血清酶Vpr在巴西路斯细菌外形成酶活性纤维,由冷EM揭示出来

Yijia Cheng¹, Jianting Han¹, Meinai Song¹

¹Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders, Ministry of Education, Shanghai Jiao Tong University, Shanghai, 200030, China.

Nature communications

|November 18, 2023

概括

研究人员发现了由细胞外酶Vpr制成的新的细菌蛋白纤维. 这些在Bacillus细菌中发现的Vpr纤维素具有酶活性,可以增强细菌的生存策略.

科学领域:

微生物学微生物学
结构生物学结构生物学
生物化学生物化学

背景情况:

细菌利用细胞外纤维结构,如鞭毛和 pili 为了生存.
细菌细胞外结构的全部范围和功能尚未完全理解.

研究的目的:

使用冷电子显微镜 (cryo-EM) 识别和描述Bacillus amyloiquefaciens中的新型细胞外蛋白纤维.
研究这些新发现的纤维的组成,组合和潜在功能.

主要方法:

使用冷电子显微镜 (cryo-EM) 可视化和分析Bacillus amyloiquefaciens的细胞外结构.
进行了生物化学测试,以确定已识别的纤维素成分的酶活性.

主要成果:

在Bacillus amyloiquefaciens周围发现了一种未报告的纤维物种,与鞭毛纤维不同.
这些新型纤维是由Vpr组成的,它是一种细胞外血清酶,在纤维组装后仍然具有酶活性.
类似的Vpr纤维在其他Bacillus物种,如Bacillus subtilis中以及在各种培养条件下观察到.

结论:

这项研究揭示了一种以前未知的细胞外纤维,该纤维由Bacillus物种中的酶活性Vpr组成.
这些Vpr纤维可能用于将化酶活动集中在细菌细胞周围,可能有助于生存或环境相互作用.
这些发现表明,在整个细菌群体中,细菌纤维的形成和功能有着保留的机制.

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